TOBRAMYCIN RESISTANCE OF PSEUDOMONAS-AERUGINOSA CELLS GROWING AS A BIOFILM ON URINARY CATHETER MATERIAL
TOBRAMYCIN RESISTANCE OF PSEUDOMONAS-AERUGINOSA CELLS GROWING AS A BIOFILM ON URINARY CATHETER MATERIAL
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DOI：
10.1128/aac.27.4.619
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发表时间：
1985-01-01
影响因子：
4.9
通讯作者：
COSTERTON, JW
中科院分区：
文献类型：
--
作者：
NICKEL, JC;RUSESKA, I;COSTERTON, JW
当导尿管材料盘暴露于含有铜绿假单胞菌细胞的动脉尿流中时，由这些细菌及其胞外多糖产物组成的厚的粘附生物膜在8小时内在乳胶表面上形成。定殖后，含有1000μg妥布霉素/ml的无菌人造尿流过该建立的生物膜，并且发现生物膜内的大部分细菌细胞在暴露于这种非常高浓度的氨基糖苷类抗生素12小时后仍然存活。就在生物膜暴露于抗生素之前从测试系统中取出的浮游(漂浮)细胞被50μg/ml妥布霉素完全杀死。当通过标准方法测定分散的细胞时，发现妥布霉素对于在导管材料定植之前从接种培养物中取出的细胞以及对于直接从妥布霉素处理的生物膜回收的存活细胞的最小抑制浓度为0.4μg/ml。厚的粘附生物膜内的生长可能赋予铜绿假单胞菌细胞一定程度的妥布霉素抗性。
When disks of urinary catheter material were exposed to the flow of arterial urine containing cells of P. aeruginosa, a thick adherent biofilm, composed of these bacteria and of their exopolysaccharide products, developed on the latex surface within 8 h. After this colonization, sterile artificial urine containing 1000 .mu.g of tobramycin/ml was flowed past this established biofilm and a significant proportion of the bacterial cells within the biofilm were found to be still viable after 12 h of exposure to this very high concentration of aminoglycoside antibiotic. Planktonic (floating) cells taken from the test system just before the exposure of the biofilm to the antibiotic were completely killed by 50 .mu.g of tobramycin/ml. The minimal inhibitory concentration of tobramycin for cells taken from the seeding cultures before colonization of the catheter material and for surviving cells recovered directly from the tobramycin-treated biofilm was found to be 0.4 .mu.g/ml when dispersed cells were assayed by standard methods. Growth within thick adherent biofilms may confer a measure of tobramycin resistance on cells of P. aeruginosa.