Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila
Roles for the Yb body components Armitage and Yb in primary piRNA biogenesis in Drosophila
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DOI：
10.1101/gad.1989510
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发表时间：
2010-11-15
影响因子：
10.5
通讯作者：
Siomi, Mikiko C.
中科院分区：
文献类型：
--
作者：
Saito, Kuniaki;Ishizu, Hirotsugu;Siomi, Mikiko C.
PIWI相互作用RNA（piRNA）保护基因组完整性免受转座子的影响。在果蝇卵巢胞体中，产生初级piRNA并加载到Piwi上。在这里，我们描述了细胞质Yb体成分Armitage和Yb在体细胞初级皮尔纳生物发生中的作用。阿米蒂奇与Piwi结合，并将Piwi定位到Yb体中。在没有Armitage或Yb的情况下，Piwi从piRNA中释放出来，不会进入细胞核。因此，皮尔纳加载是Piwi进入核所必需的。我们提出，一个功能性的Piwi-piRNA复合物的形成和检查Yb机构之前，其核进入施加转座子沉默。
PIWI-interacting RNAs (piRNAs) protect genome integrity from transposons. In Drosophila ovarian somas, primary piRNAs are produced and loaded onto Piwi. Here, we describe roles for the cytoplasmic Yb body components Armitage and Yb in somatic primary piRNA biogenesis. Armitage binds to Piwi and is required for localizing Piwi into Yb bodies. Without Armitage or Yb, Piwi is freed from the piRNAs and does not enter the nucleus. Thus, piRNA loading is required for Piwi nuclear entry. We propose that a functional Piwi-piRNA complex is formed and inspected in Yb bodies before its nuclear entry to exert transposon silencing.