Collagen species in human aorta: with special reference to basement membrane-associated collagens in the intima and media and their alteration with atherosclerosis.
Collagen species in human aorta: with special reference to basement membrane-associated collagens in the intima and media and their alteration with atherosclerosis.
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发表时间：
1987
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通讯作者：
K. Murata;C. Kotake;T. Motoyama
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作者：
K. Murata;C. Kotake;T. Motoyama
本文用十二烷基硫酸钠-聚丙烯酰胺凝胶电泳法，经微分盐沉淀和溴化氰裂解，研究了胃蛋白酶消化法提取的人主动脉胶原，特别是基膜型和短链型胶原的溶解性。基底膜相关胶原，即，IV、V和VI型胶原在较早的限制性胃蛋白酶消化中比在较晚的重复消化中更易提取，而I型胶原被持续提取。随着内膜（包括中膜）增生纤维化进程的进行，IV型胶原和V型胶原的含量增加。基底膜胶原IV型显示异质性分子大小，包括α 1（IV）和α 2（IV）链。除α 1（V）和α 2（V）链外，还发现了V型胶原的α 3（V）链。在内膜和中膜中对应于145 Kd的位置检测到短链VI型胶原，并减少至约45 Kd的片段。
Collagen species, especially basement membrane or short chain types, of human aorta extracted by limited and repeated pepsin digestions were investigated as to their type dependent solubility by sodium dodecyl sulfate-polyacrylamide gel electrophoresis after differential salt precipitation and cyanogen bromide cleavage. Basement membrane-associated collagens, i.e., types IV, V and VI, were more extractable in the earlier limited pepsin digests than in the later repeated ones, whereas type I collagen was continually extracted. The content of collagen type IV as well as that of type V increased with the proliferative fibrotic process in the intima including the media. Basement membrane collagen type IV showed heterogenous molecular sizes, including alpha 1(IV) and alpha 2(IV) chains. The alpha 3(V) chain of type V collagen was found in addition to the alpha 1(V) and alpha 2(V) chains. Short chain collagen type VI was detected in the intima and media at a position corresponding to 145 Kd and was reduced to fragments of around 45 Kd.