Development of a species-specific real-time PCR assay for diagnosing Pantoea beijingensis
Development of a species-specific real-time PCR assay for diagnosing Pantoea beijingensis
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开发用于诊断北京泛菌的物种特异性实时 PCR 检测方法
作者：
马元伟;荣成博;王守现;刘宇;王婧;许峰
近年来，北京泛菌（Pantoea beijingensis）已成为一种新发现的病原菌，可引起中国和韩国的软腐disease.in. hrpK基因是影响北京青霉致病力和毒力的关键基因之一。which.is在纯培养物中可检测到约0.1 fg的纯化质粒或3.23 × 102拷贝μl−1。此外，使用的富集程序可以诱导。100倍的灵敏度增强。本研究建立的qPCR检测方法是一种快速、可靠、灵敏的检测手段，可用于北京疫霉菌感染的早期检测，对管理实践具有一定的指导意义。
Recently, Pantoea beijingensis has become an emerging.pathogen against Pleurotus eryngii, causing soft rot disease.in China and Korea. A qPCR assay targeting hrpK, which.is predicted to be one of the key genes for the pathogenicity.and virulence of P. beijingensis, was developed and.evaluated. Approximately 0.1 fg of purified plasmid or.3.23 × 102 copies μl−1 in pure cultures could be detected..Moreover, the use of an enrichment procedure could induce.100-fold enhancement of the sensitivity. The qPCR.assay developed here is a rapid, reliable and sensitive tool.for the detection of P. beijingensis at an early stage of infection.and it will be useful in the development of management.practices.