Liquid Chromatographic Assay of Peptides Activity with Inhibiting Angiotensin Converting Enzyme
Liquid Chromatographic Assay of Peptides Activity with Inhibiting Angiotensin Converting Enzyme
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发表时间：
2010-11
期刊：
影响因子：
8.8
通讯作者：
Yu Zhi-peng Zhao Wen-zhu Lu Jing Chen Feng Liu Jing-bo-Yu-Zhi-peng-Zhao-Wen-zhu-Lu-Jing-Chen-Feng-Liu-1844064582;Lin Song-yi
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作者：
Yu Zhi-peng Zhao Wen-zhu Lu Jing Chen Feng Liu Jing-bo-Yu-Zhi-peng-Zhao-Wen-zhu-Lu-Jing-Chen-Feng-Liu-1844064582;Lin Song-yi
建立了一种快速、简便、无干扰的方法来测定鸡蛋白色蛋白水解肽对血管紧张素转换酶的抑制活性。总反应体积为60 μL，节约了成本。该测定基于合成底物马尿酸-L-组氨酰-L-亮氨酸及其水解产物马尿酸的HPLC分离和定量;在C18柱上进行分离，以体积比为25：75的乙腈/水（0. 5% TFA）的移动的流动相洗脱。在信噪比（S/N）为10时，马尿酸的检测限为（0.4600±0.0097）μmol/L。标准曲线显示线性响应，斜率为49488，相关系数为0.9995。该测定法足以研究Captopril和食物蛋白肽对ACE的抑制作用，并且与以前的方法显示出非常好的相关性。血管紧张素转换酶;高效液相色谱法;蛋清白色蛋白;肽文章ID 1005-9040（2010）-05 - 712 -05
A rapid, simple and interference-free method was developed to evaluate the inhibitory activity of hydro- lyzed peptides from egg white protein against the angiotensin-converting enzyme. The total reaction volume was 60 μL, saving the cost. The assay was based on a HPLC separation and quantification of the synthetic substrate hip- puryl-L-histidyl-L-leucine and its hydrolyzed product―hippuric acid; the separation was performed on a C18 column eluted by a mobile phase of acetonitrile/water(0.5% TFA) at a volume ratio of 25:75. At a signal to noise ratio(S/N) of 10, the detective limit of the quantitation of hippuric acid was (0.4600±0.0097) μmol/L. The standard curve shows a linear response with a slope of 49488 and a correlation coefficient of 0.9995. The assay was adequate for the study of ACE inhibition by Captopril and peptides derived from food protein, and showed a very good correlation with the previous methods. Keywords Angiotensin converting enzyme; HPLC; Egg white protein; Peptide Article ID 1005-9040(2010)-05-712-05