MicroRNA in the ovine mammary gland during early pregnancy: spatial and temporal expression of miR-21, miR-205, and miR-200
MicroRNA in the ovine mammary gland during early pregnancy: spatial and temporal expression of miR-21, miR-205, and miR-200
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DOI：
10.1152/physiolgenomics.00091.2012
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发表时间：
2013-02-01
影响因子：
4.6
通讯作者：
Devinoy, Eve
中科院分区：
文献类型：
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作者：
Galio, Laurent;Droineau, Stephanie;Devinoy, Eve
L，S，叶博亚，布迪亚夫，S，S，戴维尼。早期妊娠期间绵羊乳腺中的microRNA：miR-21，miR-205和miR-200的时空表达。PhysIOL基因组学45：151-161,2013。2012年12月26日首次出版；DOI：10.1152/Physiolgenomics.00091.2012。-从怀孕开始到哺乳期，乳腺经历了广泛的重塑；这涉及包括细胞增殖、分化和凋亡在内的细胞过程，所有这些过程都受到许多调节者的控制。为了揭示miRNA所起的作用，我们在这里描述了从怀孕早期的乳腺中克隆的47个新的绵羊miRNA，与在牛、人或小鼠中已发现的那些具有强烈的相似性。对妊娠和哺乳期成年绵羊乳腺中miRNA变化的微阵列研究表明，100miRNA受三种主要表达模式的调节：怀孕早期减少，怀孕中期达到高峰，或在妊娠晚期和哺乳期增加。通过qRT-PCR分析显示每种模式(miR-21、miR-205和miR-200b)的一个miRNA。证实了这三种miRNA在表达上的差异。应用原位杂交技术，我们检测到miR-21和miR-200在子宫腔上皮细胞中表达，而miR-205在妊娠前半期在基底层细胞中表达，在后半期在腔上皮细胞中表达。因此，我们得出结论，在妊娠早期，当细胞发生广泛的增殖时，miR-21在正常乳腺的管腔细胞中强烈表达。此外，我们还发现miR-205和miR-200在子宫腔细胞中共表达，但仅在妊娠的后半期表达。这两种miRNA可能通过抑制类似EMT的程序来协同维持上皮状态，从而实现和保存乳腺上皮细胞的分泌表型。
Galio L, Droineau S, Yeboah P, Boudiaf H, Bouet S, Truchet S, Devinoy E. MicroRNA in the ovine mammary gland during early pregnancy: spatial and temporal expression of miR-21, miR-205, and miR-200. Physiol Genomics 45: 151-161, 2013. First published December 26, 2012; doi: 10.1152/physiolgenomics.00091.2012.-The mammary gland undergoes extensive remodeling between the beginning of pregnancy and lactation; this involves cellular processes including cell proliferation, differentiation, and apoptosis, all of which are under the control of numerous regulators. To unravel the role played by miRNA, we describe here 47 new ovine miRNA cloned from mammary gland in early pregnancy displaying strong similarities with those already identified in the cow, human, or mouse. A microarray study of miRNA variations in the adult ovine mammary gland during pregnancy and lactation showed that 100 miRNA are regulated according to three principal patterns of expression: a decrease in early pregnancy, a peak at midpregnancy, or an increase throughout late pregnancy and lactation. One miRNA displaying each pattern (miR-21, miR-205, and miR-200b) was analyzed by qRT-PCR. Variations in expression were confirmed for all three miRNA. Using in situ hybridization, we detected both miR-21 and miR-200 in luminal mammary epithelial cells when expressed, whereas miR-205 was expressed in basal cells during the first half of pregnancy and then in luminal cells during the second half. We therefore conclude that miR-21 is strongly expressed in the luminal cells of the normal mammary gland during early pregnancy when extensive cell proliferation occurs. In addition, we show that miR-205 and miR-200 are coexpressed in luminal cells, but only during the second half of pregnancy. These two miRNA may cooperate to maintain epithelial status by repressing an EMT-like program, to achieve and preserve the secretory phenotype of mammary epithelial cells.