Mammalian collagenase: direct demonstration in homogenates of involuting rat uterus.
Mammalian collagenase: direct demonstration in homogenates of involuting rat uterus.
复制标题
哺乳动物胶原酶：在退化大鼠子宫匀浆中的直接演示。
DOI：
10.1016/s0006-291x(71)80170-5
复制
发表时间：
1971
影响因子：
3.1
通讯作者：
J. Woessner
中科院分区：
文献类型：
--
作者：
J. Ryan;J. Woessner
退化大鼠子宫的匀浆含有一种能够在pH 7.5和37°下消化天然胶原纤维的酶。当匀浆在6，000g下离心时，在沉淀中发现胶原酶活性和子宫胶原。在37° C下在钙存在下孵育该沉淀导致胶原蛋白的可溶性降解产物的释放。这表明胶原酶与其底物胶原纤维牢固结合，因为它不能容易地从颗粒中提取。
Homogenates of involuting rat uterus contain an enzyme capable of digesting native collagen fibers at pH 7.5 and 37°. Collagenase activity and the uterine collagen are both found in the pellet when the homogenates are centrifuged at 6,000g. Incubation of this pellet at 37° in the presence of calcium leads to the release of soluble degradation products of collagen. It is suggested that the collagenase is firmly bound to its substrate collagen fibers, since it cannot be readily extracted from the pellet.