Online Analysis of Drug Toxicity to Cells with Shear Stress on an Integrated Microfluidic Chip
Online Analysis of Drug Toxicity to Cells with Shear Stress on an Integrated Microfluidic Chip
复制标题
DOI：
10.1021/acssensors.8b01696
复制
发表时间：
2019-02-01
期刊：
影响因子：
8.9
通讯作者：
Lin, Jin-Ming
中科院分区：
文献类型：
--
作者：
Feng, Shuo;Mao, Sifeng;Lin, Jin-Ming
机械刺激，特别是流体剪切力(FSS)是细胞调节正常行为所必需的。为了更好地模拟复杂的流体微环境，迫切需要一个高通量的平台来为细胞研究提供不同的FSS。这项工作报告了一种集成的微流控芯片，它可以一致地提供五种不同的FSS梯度，以研究FSS刺激对细胞的药物毒性。与传统的提供FSS的方法相比，该装置操作更容易，吞吐量更高，并且可以消除细胞培养环境的干扰因素(唯一可变的FSS除外)。在这种多FSS平台上，药物对细胞的毒性作用比静态条件下更强烈。结果表明，FSS增强了药物的毒性。所设计的生物芯片为在更真实的微环境中评估药物的毒性提供了一个简单、高通量的平台，并有望应用于未来的药物筛选试验。
Mechanical stimulation, especially fluid shear stress (FSS), is essential for a cell to regulate regular behaviors. A high-throughput platform to provide varying FSS for cell research is desperately required for better mimicking of the complex fluidic microenvironment. This work reports an integrated microfluidic chip that could afford five different FSS gradients consistently to investigate drug toxicity on cells with the stimulation of FSS. Compared with traditional methods to provide FSS, this device would be easier to operate, have higher throughput, and could eliminate interference factors from the culture environment for cells (apart from the unique variable FSS). On such a multi-FSS platform, effects of drugs toxicity on cells were exhibited, which would be more intense than that under static conditions. The results indicated that FSS enhanced the drug toxicity. The designed biochip provides an easy and high-throughput platform to evaluate the toxicity of drugs in the more authentic microenvironment and could be promisingly applied in future drug screening tests.