State-dependent activity of neurons in the perifornical hypothalamic area during sleep and waking
State-dependent activity of neurons in the perifornical hypothalamic area during sleep and waking
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DOI：
10.1016/s0306-4522(03)00173-8
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发表时间：
2003-01-01
期刊：
影响因子：
3.3
通讯作者：
Kayama, Y
中科院分区：
文献类型：
--
作者：
Koyama, Y;Takahashi, K;Kayama, Y
含有食欲素的神经元位于穹隆周围下丘脑区域，被认为在睡眠-觉醒调节中起作用。为了研究这一区域如何参与睡眠和清醒的调节，我们记录了未服用药物、头部约束的大鼠在睡眠-唤醒周期中的神经元活动。在穹隆周围分布食欲素免疫反应神经元(PFH)的下丘脑周围区，以及PFH背侧区，包括未定带和下丘下核(统称Zi)进行记录。记录到的40个神经元分为5组：(1)异相睡眠最活跃的神经元(PS，n=14，35%)；(2)觉醒(W)和PS同时活跃的神经元(n=12，30%)；(3)W最活跃的神经元(n=7，18%)；(4)慢波睡眠最活跃的神经元(n=3，7.5%)；(5)与睡眠状态无关的神经元(n=4，10%)。在ZI记录的30个神经元中，相应的数目分别为13个(43%)、7个(23%)、6个(20%)、3个(10%)和1个(3.3%)。在这两个区域，神经元活动在PS期间的波动比在W期间更大(组3)觉醒的神经元(组3)产生的动作电位比其他类型的神经元产生的动作电位持续时间更长。在从一种状态转换到另一种状态后，大约有一半的PFH中的神经元在从一种状态转换到另一种状态后增加了它们的放电频率，而ZI中的第一和第二组神经元的百分比高于PFH中的神经元的百分比，它们在从SWS状态转换到PS状态之前增加了它们的放电速率。这些ZI神经元的放电频率在状态转移时有很大的不同。这些结果表明，PFH和ZI参与了PS或W的调节，特别是PS或维持W的时相事件的调节。在PS的诱导或与PS相关的某些时相现象中，ZI似乎比PFH更密切地参与。(C)2003年IBRO。爱思唯尔科学有限公司出版。版权所有。
Neurons containing orexins are located in the perifornical hypothalamic area and are considered to have a role in sleep-wake regulation. To examine how this area is involved in the regulation of sleep and wakefulness, we recorded neuronal activity in undrugged, head-restrained rats across sleep-waking cycles. Recordings were made in the perifornical hypothalamic area where orexin-immunoreactive neurons are distributed (PFH), and in the area dorsal to the PFH, including the zona incerta and subincertal nucleus (collectively referred to as ZI). The 40 neurons recorded from in the PFH were divided into five groups: (1) neurons most active during paradoxical sleep (PS, n=14, 35%), (2) neurons active during both waking (W) and PS (n=12, 30%), (3) neurons most active during W (n=7, 18%), (4) neurons most active during slow-wave sleep (SWS, n=3, 7.5%), and (5) neurons whose activity had no correlation with sleep-waking states (n=4, 10%). Of 30 neurons recorded from in the ZI, the corresponding numbers were 13 (43%), seven (23%), six (20%), three (10%), and one (3.3%). In both areas, neuronal activity fluctuated more during PS than during W. Waking-specific neurons (group 3) in the PFH generated action potentials with longer durations than those produced by other types of neurons. About half of the neurons in the PFH that were classified in groups 1, 2, and 3 increased their firing rate after the transition from one state to another, while higher percentages of neurons of groups 1 and 2 in the ZI than those in the PFH increased their firing rate prior to the state shift from SWS to PS. In these ZI neurons, however, the firing rate varied considerably at the state shift.These results suggest that the PFH and ZI are involved in the regulation of PS or W, especially the regulation of phasic events during PS or the maintenance of W. The ZI appears to be more closely involved than the PFH in the induction of PS or some phasic phenomena associated with PS. (C) 2003 IBRO. Published by Elsevier Science Ltd. All rights reserved.