Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm
Interferometric time-stretch microscopy for ultrafast quantitative cellular and tissue imaging at 1 μm
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DOI：
10.1117/1.jbo.19.7.076001
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发表时间：
2014-07-01
影响因子：
3.5
通讯作者：
Tsia, Kevin K.
中科院分区：
文献类型：
--
作者：
Lau, Andy K. S.;Wong, Terence T. W.;Tsia, Kevin K.
定量相位成像（QPI）已被证明是生物标本无标记表征的有力工具。然而，成像速度在很大程度上受到图像传感器技术的限制，阻碍了其在高通量筛选和高效大数据分析的应用中的应用。我们在这里展示了干涉时间拉伸（iTS）显微镜，用于提供超快速定量相细胞和组织成像，成像线扫描速率bbb20 mhz -比传统QPI快几个数量级。在1 μ m波长窗口内实现高效的时间拉伸操作，我们提出了一种iTS显微镜系统，用于固定细胞和组织切片的实际超快QPI，以及超快流动细胞（流速高达8 m/s）。据我们所知，这是时间拉伸成像首次能够以纳米精度揭示细胞和组织的定量形态信息。由于许多参数可以从相中进一步提取，并且可以作为疾病诊断的内在生物标志物，iTS显微镜可以在高通量和高含量的细胞分析（例如成像流式细胞术）以及组织折射成像（例如数字病理学的全片成像）中找到自己的位置。(C) 2014年中国光学仪器工程师学会（SPIE）
Quantitative phase imaging (QPI) has been proven to be a powerful tool for label-free characterization of biological specimens. However, the imaging speed, largely limited by the image sensor technology, impedes its utility in applications where high-throughput screening and efficient big-data analysis are mandated. We here demonstrate interferometric time-stretch (iTS) microscopy for delivering ultrafast quantitative phase cellular and tissue imaging at an imaging line-scan rate >20 MHz-orders-of-magnitude faster than conventional QPI. Enabling an efficient time-stretch operation in the 1-mu m wavelength window, we present an iTS microscope system for practical ultrafast QPI of fixed cells and tissue sections, as well as ultrafast flowing cells (at a flow speed of up to 8 m/s). To the best of our knowledge, this is the first time that time-stretch imaging could reveal quantitative morphological information of cells and tissues with nanometer precision. As many parameters can be further extracted from the phase and can serve as the intrinsic biomarkers for disease diagnosis, iTS microscopy could find its niche in high-throughput and high-content cellular assays (e. g., imaging flow cytometry) as well as tissue refractometric imaging (e.g., whole-slide imaging for digital pathology). (C) 2014 Society of Photo-Optical Instrumentation Engineers (SPIE)