Nodulation and N2 fixation effectiveness of Bradyrhizobium strains in symbiosis with Adzuki Bean, Vigna angularis
Nodulation and N2 fixation effectiveness of Bradyrhizobium strains in symbiosis with Adzuki Bean, Vigna angularis
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DOI：
10.1590/s1516-89132010000200007
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发表时间：
2010-04-01
影响因子：
1
通讯作者：
Miličić, Bogić
中科院分区：
文献类型：
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作者：
Delić, Dušica;Stajković, Olivera;Miličić, Bogić
在盆栽实验中，从塞尔维亚土壤中分离出一种菌株 Knj、四种慢生根瘤菌菌株和三种慢生根瘤菌菌株。研究了对红小豆结瘤的影响和共生 N-2 固定的有效性。所有受试菌株均在小豆中产生根瘤。 B. japonicum 菌株表现出很高的 N-2 固定潜力，特别是 525 和 542。B. japonicum 菌株产生的芽干重为 65-71%，总氮含量为未接种对照（全氮含量（100%））的 99-138%。接种慢生根瘤菌属的植物之间没有发现显着差异。菌株和未接种的对照植物不含氮（茎干重分别为 40-42% 和 42%），这表明慢生根瘤菌属共生 N-2 固定失活。菌株。 Knj 菌株处于中间位置（芽干重的 56%）。这些数据表明，B. japonicum 525和542菌株可用于进一步研究，以便将它们用作微生物氮肥中的接种剂。
In pot experiment, one isolate Knj from a Serbian soil, four strains of Bradyrhizobium japonicum and three strains of Bradyrhizobium spp. were examined for the effect on adzuki bean nodulation and effectiveness in symbiotic N-2 fixation. All the tested strains produced root nodules in adzuki bean. Strains of B. japonicum showed high potential of N-2 fixation, particularly 525 and 542. B. japonicum strains resulted 65-71% shoot dry weight and 99-138% total N content of uninoculated control with full N content (100%). No significant difference was found between the plants inoculated with Bradyrhizobium spp. strains and uninoculated control plants without N (40-42 and 42% shoot dry weight, respectively), which indicated symbiotic N-2 fixation inactivity of the Bradyrhizobium spp. strains. Knj strain had the middle position (56% shoot dry weight). These data showed that B. japonicum 525 and 542 strains could be used in further investigations in order to apply them as inoculants in microbiological N fertilizers.