An endogenous ovarian growth factor which stimulates BALB/3T3 and granulosa cell proliferation.
An endogenous ovarian growth factor which stimulates BALB/3T3 and granulosa cell proliferation.
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一种内源性卵巢生长因子，可刺激 BALB/3T3 和颗粒细胞增殖。
作者：
A. Makris;M. Klagsbrun;T. Yasumizu;K. Ryan
猪全卵巢提取物对BALB/3T3细胞DNA合成的促进作用生化特性表明，生长因子与一种对热不稳定、对二硫苏糖醇处理稳定的可溶性阳离子蛋白有关，并从Sephadex G-100上洗脱，介于糜蛋白酶(25,000 mR)和细胞色素C(12,700 mR)之间。膜组织提取物对颗粒细胞和3T3细胞均有促生长活性。来自膜细胞而不是颗粒细胞的条件培养液刺激静止的3T3和颗粒细胞的[~3H]DNA合成。结果表明，卵泡膜中存在一种内源性蛋白卵巢生长因子，可在体外刺激颗粒细胞的增殖。
Extracts of whole porcine ovary stimulate BALB/3T3 [3H] DNA synthesis in BALB/3T3 cells. Biochemical characterization indicates the growth factor is associated with a soluble cationic protein that is labile to heat, stable to dithiothreitol treatment and elutes from Sephadex G-100 between chymotrypsin (25,000 Mr) and cytochrome C (12,700 Mr). Extracts of thecal tissue have growth-promoting activity in both granulosa and 3T3 cells. Conditioned medium from thecal but not granulosa cell cultures stimulates [3H] DNA synthesis in quiescent 3T3 and granulosa cells. The results indicate the presence of an endogenous protein ovarian growth factor found in the theca that stimulates granulosa cell proliferation in vitro.