NMR assignment of human ubiquitin conjugating enzyme Ubc7
NMR assignment of human ubiquitin conjugating enzyme Ubc7
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DOI：
10.1007/s10858-005-1257-7
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发表时间：
2005-08-01
影响因子：
2.7
通讯作者：
Tolman, JR
中科院分区：
文献类型：
--
作者：
Briggman, KB;Majumdar, A;Tolman, JR
疟疾寄生虫恶性疟原虫（Plasmodium falciparum）在人红细胞中度过其生命周期的一部分，在那里它受到增强的氧化应激的挑战，并且在那里它需要有效的抗氧化剂来维持其还原环境并保护自身免受损害（Rahlfs等人，2002年）。硫氧还蛋白（PfTrx）是主要的氧化还原调节分子之一，由于其二硫醇/二硫键交换活性，其决定了蛋白质半胱氨酸的氧化态，因此是抗疟疾药物研究中的潜在靶标（Kanzok等人，2000年）。我们开始的重组104-氨基酸多肽的结构测定，使用多维NMR光谱。在HNCA、CBCA（CO）NH、CBCANH、HNCO、1H）15 N-HSQC和1H）15 N-NOESY-HSQC实验的基础上进行连续骨架共振归属。使用HCCH-TOCSY实验，在2 H2O中对样品进行侧链分配。对PfTrx的99%的主链1HN、13 Ca 15 NH（不考虑脯氨酸残基）、89%的13 C ′、99%的1Ha和88%的侧链原子（芳环的13 C位移除外）进行了化学位移归属。数据以登录号6442保藏在BioMagResBank中。参考文献：Kanzok et al.（2000）生物化学杂志，275，40181-40186; Rahlfs等人等人（2002）Cell.摩尔生命科学，59，1024-1041。
The malaria parasite Plasmodium falciparum spends part of its life cycle in human erythrocytes, where it is challenged with enhanced oxidative stress, and where it needs efficient anti-oxidants to maintain its reducing milieu and to protect itself against damage (Rahlfs et al., 2002). Thioredoxin (PfTrx) is one of the major redoxregulatory molecules which, because of its dithiol/disulfide exchange activity, determines the oxidation state of protein cysteines, being therefore a potential target in research towards anti-malarial drugs (Kanzok et al., 2000). We initiated the structure determination of the recombinant 104-amino acid polypeptide, using multidimensional NMR spectroscopy. Sequential backbone resonance assignments were carried out on the basis of HNCA, CBCA (CO) NH, CBCANH, HNCO, 1H) 15N-HSQC and 1H) 15N-NOESY-HSQC experiments. Side-chain assignments were done using the HCCH-TOCSY experiment with the sample in 2H2O. The chemical shifts for 99% of the backbone1HN, 13Ca 15NH (without considering the proline residues), for 89% of the 13C′, 99% of the 1Ha, and 88% of all side-chain atoms (except the 13C shifts of the aromatic rings) of the PfTrx were assigned. Data were deposited in the BioMagResBank under accession number 6442. References: Kanzok et al.(2000) J. Biol. Chem., 275, 40181–40186; Rahlfs et al.(2002) Cell. Mol. Life Sci., 59, 1024–1041.