Establishment of a ternary network system for evaluating the antioxidant fraction of Danhong injection
Establishment of a ternary network system for evaluating the antioxidant fraction of Danhong injection
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丹红注射液抗氧化成分评价三元网络体系的建立
DOI：
10.1002/bmc.3739
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发表时间：
2016-10-01
影响因子：
1.8
通讯作者：
Wang, Yuefei
中科院分区：
文献类型：
--
作者：
Wang, Yan;Jiang, Zhenzuo;Wang, Yuefei
氧化应激在许多心血管疾病中起着至关重要的作用。丹红注射液是一种有效的治疗心血管疾病的药物，被认为是通过抗氧化机制发挥作用的。在我们的研究中，我们专注于DHI组分（Frs）的抗氧化能力的潜在贡献，并建立了一个创新的筛选方法的基础上，2，2-二苯基-1-苦肼自由基清除活性测定。以清除自由基活性、活性-浓度曲线下面积和固形物含量为评价指标，构建三元网络评价体系，筛选出抗氧化效果最好的组分。结果，根据三元网络的回归面积，Frs 5-7和Frs 17-19显示出表现出上级抗氧化活性，其>0.5。采用超高效液相色谱-四极杆飞行时间质谱-核磁共振联用技术对活性组分进行了表征。该研究为综合评价DHI各组分的抗氧化作用提供了有效方法。版权所有（c）2016约翰威利父子有限公司
Oxidative stress plays a crucial role in numerous cardiovascular diseases. As an effective therapy, Danhong injection (DHI) is considered to act through an antioxidant mechanism for the treatment of cardiovascular disease. In our study, we focused on the potential contribution of the antioxidant capacity of DHI fractions (Frs) and established an innovative screening method based on a 2,2-diphenyl-1-picrylhydrazyl radical scavenging activity assay. A ternary network evaluation system, which was constructed based on the radical scavenging activity, the area under the activity-concentration curve and the solid content of the fractions, was implemented to select the fractions that posed the greatest antioxidant effect. As a result, Frs 5-7 and Frs 17-19 were shown to exhibit superior antioxidant activity according to the regression area of the ternary network, which was >0.5. Furthermore, the active fractions were characterized by ultra-performance liquid chromatography coupled with quadrupole time-of-flight mass spectrometry combined with nuclear magnetic resonance. This study provided an effective method for the comprehensive evaluation of the antioxidant effect of DHI fractions. Copyright (c) 2016 John Wiley & Sons, Ltd.