A Novel Micro Cold Atmospheric Plasma Device for Glioblastoma Both In Vitro and In Vivo.
A Novel Micro Cold Atmospheric Plasma Device for Glioblastoma Both In Vitro and In Vivo.
复制标题
DOI：
10.3390/cancers9060061
复制
发表时间：
2017-05-30
期刊：
影响因子：
5.2
通讯作者：
Keidar M
中科院分区：
文献类型：
--
作者：
Chen Z;Simonyan H;Cheng X;Gjika E;Lin L;Canady J;Sherman JH;Young C;Keidar M
冷常压等离子体（CAP）治疗是一项迅速发展和新兴的癌症治疗技术。直接CAP喷射照射仅限于皮肤，它也可以作为手术期间的补充治疗，因为它只会导致上面三到五个细胞层的细胞死亡。然而，目前的等离子体发射管对于颅内应用来说太大了。为了提高CAP方法在脑肿瘤治疗中的效率和适用性，减小等离子体射流的气体流速和尺寸，研制了一种新型微型CAP装置（µCAP），并将其应用于小鼠脑胶质母细胞瘤肿瘤的治疗。应用各种等离子体诊断技术来评估氦µCAP的物理特性，如电子密度、放电电压和光学发射光谱（OES）。在体外研究了µCAP对胶质母细胞瘤（U87MG-RedFluc）癌细胞的直接和间接影响。结果表明，µCAP产生短寿命和长寿命的物种和自由基（即羟基自由基（•OH）、过氧化氢（H2O2）和亚硝酸盐（NO2−）等），并以剂量依赖的方式增加肿瘤细胞的死亡。将这些发现转化为体内环境，表明颅内µCAP可有效阻止小鼠脑内胶质母细胞瘤肿瘤的生长。µCAP装置可以安全地用于小鼠，从而抑制肿瘤生长。这些初步观察结果表明，µCAP装置在胶质母细胞瘤治疗中可能是一种有用的消融治疗工具。
Cold atmospheric plasma (CAP) treatment is a rapidly expanding and emerging technology for cancer treatment. Direct CAP jet irradiation is limited to the skin and it can also be invoked as a supplement therapy during surgery as it only causes cell death in the upper three to five cell layers. However, the current cannulas from which the plasma emanates are too large for intracranial applications. To enhance efficiency and expand the applicability of the CAP method for brain tumors and reduce the gas flow rate and size of the plasma jet, a novel micro-sized CAP device (µCAP) was developed and employed to target glioblastoma tumors in the murine brain. Various plasma diagnostic techniques were applied to evaluate the physics of helium µCAP such as electron density, discharge voltage, and optical emission spectroscopy (OES). The direct and indirect effects of µCAP on glioblastoma (U87MG-RedFluc) cancer cells were investigated in vitro. The results indicate that µCAP generates short- and long-lived species and radicals (i.e., hydroxyl radical (•OH), hydrogen peroxide (H2O2), and nitrite (NO2−), etc.) with increasing tumor cell death in a dose-dependent manner. Translation of these findings to an in vivo setting demonstrates that intracranial µCAP is effective at preventing glioblastoma tumor growth in the mouse brain. The µCAP device can be safely used in mice, resulting in suppression of tumor growth. These initial observations establish the µCAP device as a potentially useful ablative therapy tool in the treatment of glioblastoma.