IMMOBILIZATION OF BIOCATALYSTS WITH BOMBYX-MORI SILK FIBROIN BY SEVERAL KINDS OF PHYSICAL TREATMENT AND ITS APPLICATION TO GLUCOSE SENSORS
IMMOBILIZATION OF BIOCATALYSTS WITH BOMBYX-MORI SILK FIBROIN BY SEVERAL KINDS OF PHYSICAL TREATMENT AND ITS APPLICATION TO GLUCOSE SENSORS
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DOI：
10.1016/0265-928x(89)80002-1
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发表时间：
1989-01-01
期刊：
影响因子：
--
通讯作者：
KUROO, T
中科院分区：
文献类型：
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作者：
DEMURA, M;ASAKURA, T;KUROO, T
制备了固定化生物催化剂的桑蚕丝素膜。水溶性膜的不溶解化仅通过物理处理进行，即在较高湿度下拉伸、压缩和静置以及甲醇浸渍处理，而不使用任何共价结合试剂。进行的所有物理处理对于将酶固定在膜中的目的是有效的。对葡萄糖氧化酶（GOD）固定化膜的结构进行了详细的表征。衬底的渗透性取决于晶体结构，即，膜的丝蛋白I和丝蛋白II的分数。固定化GOD的酶活产率为游离酶活的80%以上，但随GOD浓度的增加而降低。这似乎是由于基板的扩散限制。固定化酶的pH稳定性和热稳定性都有很大提高，且与固定化方法基本无关。介绍了葡萄糖氧化酶（GOD）或微生物荧光假单胞菌固定化丝素膜作为葡萄糖传感器的研究进展。
Biocatalyst-immobilized Bombyx mori silk fibroin membrane was prepared. The insolubilization of the water-soluble membranes was performed by physical treatments only, i.e. stretching, compressing and standing under higher humidity and methanol-immersion treatment, without any use of covalently binding reagent. All physical treatments performed were effective for the purpose of the immobilization of the enzymes in the membranes. The structural characterization of the glucose oxidase (GOD) immobilized membrane was performed in detail. The permeability of the substrate depends on the crystalline structure, i.e., the fraction of Silk I and Silk II of the membrane. The activity yield of the immobilized GOD was more than 80% of the value of free enzyme when 0.002% of the enzyme was entrapped in the membrane, but it decreased with increasing the concentration of the GOD in the membrane. This seems to result from diffusion limitation of the substrate. The pH and thermal stabilities of the immobilized enzyme were much improved, and were essentially independent of the methods of the immobilization. Development of the GOD or microorganism, Pseudomonas fluorescens immobilized silk fibroin membranes as glucose sensors are described.