Sox-2 in taste bud and lateral line system of zebrafish during development
Sox-2 in taste bud and lateral line system of zebrafish during development
复制标题
DOI：
10.1016/j.neulet.2009.09.056
复制
发表时间：
2009-12-18
影响因子：
2.5
通讯作者：
Ciriaco, E.
中科院分区：
文献类型：
--
作者：
Germana, A.;Montalbano, G.;Ciriaco, E.
SOX-2是一种转录因子，参与包括鱼类在内的不同脊椎动物的成体神经发生。SOX-2还参与鱼类侧线系统神经肥大细胞的生长和更新，对哺乳动物味蕾的发育是必不可少的。应用免疫组织化学和免疫印迹技术，对斑马鱼受精后10d至成鱼(1年)的SOX-2味蕾和神经肥大的发生和定位进行了研究。所使用的抗体识别出两条蛋白质带，估计分子量分别为34和37 kDa，与预测的SOX-2一致。在所有年龄段中，味蕾中的感觉细胞均呈SOX-2免疫反应阳性，而在神经肥大细胞中，SOX-2的表达仅限于基底非感觉细胞。有趣的是，在与味蕾和神经肥大相关的上皮细胞中观察到了SOX-2免疫反应。目前的研究结果表明，SOX-2在斑马鱼的整个生命周期中都在味蕾和神经母细胞中表达。然而，虽然SOX-2在斑马鱼味蕾中的作用仍未确定，但在神经肥大中的结果表明，SOX-2可能参与机械感觉细胞的细胞更新。(C)2009爱思唯尔爱尔兰有限公司。保留所有权利。
The Sox-2 is a transcription factor involved in adult neurogenesis in different vertebrate species, including fishes. Sox-2 also participates in growth and renewal on sensory cells in neuromasts of the fish lateral line system, and it is essential for development of taste buds in mammals. Using immunohistochemistry and Western blot we have investigated the occurrence and localization of Sox-2 taste buds and neuromast of zebrafish from 10 days post-fertilization to adult stage (1 year). The antibody used identifies two protein bands with estimated molecular weights of 34 and 37 kDa which are consistent with those predicted for Sox-2. Sensory cells in taste buds displayed Sox-2 immunoreactivity at all the ages sampled, whereas in the neuromasts Sox-2 expression was restricted to the basal non-sensory cells. Interestingly Sox-2 immunoreactivity was observed in epithelial cells associated with both taste buds and neuromasts. Present results demonstrate that Sox-2 expressed in taste buds and neuromasts of zebrafish during the whole lifespan. Nevertheless, whereas the role of Sox-2 in taste buds of zebrafish remains to be established, the results in neuromast suggest that Sox-2 could participate in cell renewal of the mechanosensory cells. (C) 2009 Elsevier Ireland Ltd. All rights reserved.