SCANNING AND TRANSMISSION ELECTRON-MICROSCOPIC STUDIES OF JEJUNAL MICROVILLI OF RAT, HAMSTER AND DOG
SCANNING AND TRANSMISSION ELECTRON-MICROSCOPIC STUDIES OF JEJUNAL MICROVILLI OF RAT, HAMSTER AND DOG
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DOI：
10.1002/jmor.1051410304
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发表时间：
1973-01-01
影响因子：
1.5
通讯作者：
TAYLOR, AB
中科院分区：
文献类型：
--
作者：
ANDERSON, JH;TAYLOR, AB
用扫描和透射电子显微镜观察了大鼠、仓鼠和犬空肠绒毛上皮细胞的微绒毛表面积和单位面积微绒毛数目，计算出大鼠、仓鼠和狗的平均微绒毛表面积分别为0.419μ2、0.573μ2和0.751μ2。大鼠每平方米冻干绒毛表面微绒毛最多，平均值为65。仓鼠和狗的冻干标本的微绒毛平均值分别为54和34，每平方米微米绒毛表面的微绒毛总面积与鼠类、仓鼠和犬类的相关性更密切，分别为27.23、30.94和25.53。这些数据表明，所研究物种的平均微绒毛表面积与种群密度呈负相关。然而，这三个物种单位绒毛表面的微绒毛总面积相对相似。观察到的单位绒毛表面微绒毛的数量因制备扫描电子显微镜样品所采用的脱水技术而异。这种变化可能反映了收缩伪影，在涉及扫描电子显微镜的软组织研究中应予以考虑。
Scanning and transmission electron microscopy were used to investigate the surface area and number per unit area of microvilli from jejunal villus epithelial cells in the rat, hamster and dog.The calculated mean microvillus surface area was 0.419 μ2, 0.573 μ2, 0.751 μ2for the rat, hamster and dog respectively.The largest number of microvilli per square micron freeze dried villus surface was measured in the rat with a mean value of 65. Hamster and dog freeze dried specimens had lower mean values of 54 and 34 microvilli respectively.The total microvilli surface area in square micron per square micron villus surface was more closely related for the three species with values of 27.23 for the rat, 30.94 for the hamster and 25.53 for the dog.These data indicate an inverse relationship between the mean microvillus surface area and population density in the species studied. However, the total microvilli surface area per unit villus surface is relatively similar for the three species.The observed number of microvilli per unit villus surface was shown to vary depending upon the dehydration technique employed for preparation of scanning electron microscopic specimens. This variation probably reflects shrinkage artifact and should be considered in soft tissue studies involving the scanning electron microscope.