Simultaneous molecular and hypoxia imaging of brain tumors in vivo using spectroscopic photoacoustic tomography
Simultaneous molecular and hypoxia imaging of brain tumors in vivo using spectroscopic photoacoustic tomography
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DOI：
10.1109/jproc.2007.913515
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发表时间：
2008-03-01
影响因子：
20.6
通讯作者：
Wang, Lihong V.
中科院分区：
文献类型：
--
作者：
Li, Meng-Lin;Oh, Jung-Taek;Wang, Lihong V.
体内无创分子与功能成像在检测和监测动物乃至最终人类的各种生理状况方面很有前景。为此，我们提出了一种新型无创技术——光谱光声断层成像（SPAT），它兼具强光学吸收对比度和高超声空间分辨率。光学对比度能够对多种光吸收物质（例如，氧合血红蛋白、去氧血红蛋白以及一种分子造影剂）的信号贡献进行光谱分离，从而实现同时进行分子与功能成像。SPAT以高分辨率成功地对靶向在裸鼠脑内人U87胶质母细胞瘤中过表达的整合素α(v)β(3)的一种分子造影剂的分布进行了成像。同时，SPAT还对血管系统的血红蛋白氧饱和度以及总血红蛋白浓度进行了成像，这揭示了肿瘤新生血管中的缺氧情况。因此，SPAT有可能促使人们更好地理解血液动力学与肿瘤进展相关的特定生物标志物之间的相互关系。
Noninvasive molecular and functional imaging in vivo is promising for detecting and monitoring various physiological conditions in animals and ultimately humans. To this end, we present a novel noninvasive technology, spectroscopic photoacoustic tomography (SPAT), which offers both strong optical absorption contrast and high ultrasonic spatial resolution. Optical contrast allows spectroscopic separation of signal contributions from multiple optical absorbers (e.g.,, oxyhemoglobin, deoxyhemoglobin, and a molecular contrast agent), thus enabling simultaneous molecular and functional imaging. SPAT successfully imaged with high resolution the distribution of a molecular contrast agent targeting integrin alpha(v)beta(3) overexpressed in human U87 glioblastomas in nude mouse brains. Simultaneously, SPAT also imaged the hemoglobin oxygen saturation and the total hemoglobin concentration of the vasculature, which revealed hypoxia in tumor neovasculature. Therefore, SPAT can potentially lead to better understanding of the interrelationships between hemodynamics and specific biomarkers associated with tumor progression.