Poststimulation Block of Pudendal Nerve Conduction by High-Frequency (kHz) Biphasic Stimulation in Cats
Poststimulation Block of Pudendal Nerve Conduction by High-Frequency (kHz) Biphasic Stimulation in Cats
复制标题
DOI：
10.1111/ner.13060
复制
发表时间：
2020-08-01
期刊：
影响因子：
2.8
通讯作者：
Tai, Changfeng
中科院分区：
文献类型：
--
作者：
Wang, Zhaoxia;Pace, Natalie;Tai, Changfeng
目的探讨高频双相电刺激(HFBS)各参数与猫阴部神经高频双相刺激后恢复时间的关系。材料与方法在10只猫的阴部神经上植入三极袖带电极以输送HFBS。将两个钩电极放置在袖带电极的中央或远端，以刺激阴部神经并诱发外尿道括约肌收缩。向远端尿路插入导尿管，缓慢地对尿路进行灌流，并记录EUS收缩产生的支持压力。在确定阻断阈值(T)后，使用不同持续时间(1、5、10、20、30min)和不同强度(1Tor 2T)的HFBS(6或10 kHz)制作HFBS后阻断。结果10 kHz、1T强度的HFBS至少需要30min才能诱导HFBS后的阻断。而高强度(2T)10 kHz HFBS仅刺激10min即可引起HFBS后的阻滞，而以2T的10 kHz HFBS刺激30min则可引起较长时间(1~3h)的HFBS后阻滞，并随着时间的推移完全恢复。6 kHz持续5min的HFBS所致的HFBS后阻滞时间(20.4±2.1min，p<；0.05，N=5只猫)明显长于10 kHz的HFBS(9.5±2.1min)。结论持续时间较长、强度较高、频率较低的HFBS可产生较持久的可逆性HFBS后阻滞。本研究对开发HFBS阻断神经传导的新方法具有重要意义。
Objective To determine the relationship between various parameters of high-frequency biphasic stimulation (HFBS) and the recovery period of post-HFBS block of the pudendal nerve in cats. Materials and Methods A tripolar cuff electrode was implanted on the pudendal nerve to deliver HFBS in ten cats. Two hook electrodes were placed central or distal to the cuff electrode to stimulate the pudendal nerve and induce contractions of external urethral sphincter (EUS). A catheter was inserted toward the distal urethra to slowly perfuse the urethra and record the back-up pressure generated by EUS contractions. After determining the block threshold (T), HFBS (6 or 10 kHz) of different durations (1, 5, 10, 20, 30 min) and intensities (1Tor 2T) was used to produce the post-HFBS block. Results HFBS at 10 kHz and 1Tintensity must be applied for at least 30 min to induce post-HFBS block. However, 10 kHz HFBS at a higher intensity (2T) elicited post-HFBS block after stimulation of only 10 min; and 10 kHz HFBS at 2Tfor 30 min induced a longer-lasting (1-3 h) post-HFBS block that fully recovered with time. HFBS of 5-min duration at 6 kHz produced a longer period (20.4 +/- 2.1 min,p < 0.05,N= 5 cats) of post-HFBS block than HFBS at 10 kHz (9.5 +/- 2.1 min). Conclusion HFBS of longer duration, higher intensity, and lower frequency can produce longer-lasting reversible post-HFBS block. This study is important for developing new methods to block nerve conduction by HFBS.