Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer.
Assay of matrix metalloproteinases types 1, 2, 3 and 9 in breast cancer.
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乳腺癌中1、2、3和9类型基质金属蛋白酶的测定。
DOI：
10.1038/bjc.1998.153
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发表时间：
1998-03
影响因子：
8.8
通讯作者：
Duffy, M J
中科院分区：
文献类型：
--
作者：
Remacle, A G;Noel, A;Duggan, C;McDermott, E;O'Higgins, N;Foidart, J M;Duffy, M J
基质金属蛋白酶（MMPs）是锌依赖性的内肽酶，与癌症的侵袭和转移有关。对84例人乳腺癌组织和7例正常乳腺组织进行了明胶酶谱分析。在11个（12%）样品中发现了72 kDa的MMP-2前体形式，而在3个（6%）和34个（40%）样品中分别发现了62 kDa和59 kDa的两种活化形式。与MMP-2相比，大多数样品（52%）含有前体形式的MMP-9。使用ELISA法，12%的样品中检测到MMP-1水平，而仅2%的样品中检测到MMP-3水平。MMP-2、-3和-9的水平与淋巴结转移数量呈负相关。MMP-2和-9水平与患者预后均无显著相关性。然而，经酶谱分析，高水平的50 kda明胶酶带患者的生存率明显高于低水平患者。在正常乳腺组织中从未观察到该物种。
Matrix metalloproteinases (MMPs) are zinc dependent endopeptidases implicated in cancer invasion and metastasis. Gelatin zymography was performed on 84 human breast carcinomas and seven normal breast tissues. The precursor form of MMP-2 (72 kDa) was found in 11 (12%) samples, while its two activated forms, i.e. 62 kDa and 59 kDa, were found in three (6%) and 34 (40%) samples respectively. In contrast to MMP-2, most of the samples (52%) contained MMP-9 in its precursor form. Using ELISA, MMP-1 levels were found in 12% of the samples while MMP-3 levels were found in only 2% of the samples. Levels of MMP-2, -3 and -9 correlated inversely with numbers of nodal metastases. Neither MMP-2 nor -9 levels were significantly related to patient outcome. However, patients with high levels of a 50-kDa gelatinase band after zymography had a significantly better survival than patients with low levels. This species was never observed in normal breast tissue.