Electrochemical Impedance Simulation for Single Cell Analysis using a Microelectrode
Electrochemical Impedance Simulation for Single Cell Analysis using a Microelectrode
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DOI：
10.5220/0010266401140120
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发表时间：
2020-01
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影响因子：
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通讯作者：
Masataka Shiozawa;S. Uno
中科院分区：
文献类型：
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作者：
Masataka Shiozawa;S. Uno
电化学阻抗谱(EIS)是从细胞形态和细胞-底物粘附性方面监测活细胞的方法之一。在传统的电池分析中，获得的是大面积电极上多个电池的平均值。同时，利用小于单个电池尺寸的微电极获得了单个电池的特性。在这项研究中，我们通过计算机模拟研究了电极尺寸对单电池EIS分析的影响。小于电池尺寸的电极可以独立测量细胞质的电导率和细胞-衬底的间隙电阻。此外，还应在不同的频率范围内同时监测细胞高度和半径等形态的变化。因此，我们的模拟表明，微尺度电极的EIS测量可以用于监测细胞的各种生理变化，如免疫反应。
Electrochemical impedance spectroscopy (EIS) is one of the ways to monitor living cells in terms of morphology and cell-substrate adhesion. In conventional cell analysis, averaged values from many cells on a large area electrode are acquired. Meanwhile, single cell characteristics are obtained by using a microelectrode which is smaller than single cell size. In this study, we investigated the impact of electrode size on single cell EIS analysis by using a computer simulation. The electrode smaller than the cell size would enable independent measurement of the cytoplasm electrical conductivity and the cell-substrate gap resistance. In addition, changes in morphology such as cell height and radius should be monitored at the same time in different frequency range. Thus, our simulation indicates that EIS measurement with microscale electrode may be used in monitoring various physiological changes in cells such as immune response.