Adsorption of endotoxin by beta2-microglobulin adsorbent column (Lixelle): the new approach for endotoxinemia.
Adsorption of endotoxin by beta2-microglobulin adsorbent column (Lixelle): the new approach for endotoxinemia.
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β2-微球蛋白吸附柱（Lixelle）吸附内毒素：治疗内毒素血症的新方法。
DOI：
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
T. Nakatani
中科院分区：
文献类型：
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作者：
K. Tsuchida;Y. Takemoto;K. Sugimura;R. Yoshimura;Keisuke Yamamoto;T. Nakatani
我们以前报道过，用于β 2-微球蛋白（BMG）吸附柱的Lixelle不仅可以吸附BMG，还可以吸附炎性细胞因子。我们对力赛在内毒素血症患者中的应用产生了兴趣，并使用脂多糖（LPS）（大肠杆菌B8）、内毒素（ET）污染的水研究了其体外吸附微生物成分的能力。每种水溶液的初始浓度为LPS（ET 29，135 EU/L），污染水（ET 3，523 EU/L）全血溶液的初始浓度为LPS（ET 1，197.6 EU/L）。每2.5 ml储备溶液和调整后的稀释溶液含有0.5 ml Lixelle珠。在37 ℃下振荡2 h后，使用内毒素特异性鲎试剂法测定溶液中的ET。结果表明，即使在LPS和污染的水溶液中孵育的ET浓度和全血中的高，含有Lixelle珠的样品显示出显着降低。因此，Lixelle珠不仅可以吸附BMG，还可以吸附微生物组分，如LPS和ET。这些发现与Lixelle吸附ET的能力一起显示了应用于治疗内毒素血症的可能性。
We previously reported that Lixelle, which was used for beta2-microglobulin (BMG) adsorption columns, could adsorb not only BMG but also inflammatory cytokines. We became interested in the application of Lixelle for patients with endotoxinemia and researched its ability to adsorb microorganism components in vitro using lipopolysaccharide (LPS) (Escherichia coli B8), endotoxin (ET) contaminated water. The initial concentrations of each water solution were LPS (ET 29,135 EU/L) and contaminated water (ET 3,523 EU/L) whole blood solution was LPS (ET 1,197.6 EU/L). Each 2.5 ml of the stock solution and adjusted diluted solutions contained 0.5 ml of Lixelle beads. After shaking at 37 degrees C for 2 h, ET in the solutions was determined using the endotoxin specific-limulus amebocyte lysate method. The results revealed that even though ET concentrations in LPS and contaminated water incubated in water solution and in whole blood were high, the samples containing Lixelle beads showed significant decreases. Thus, Lixelle beads can adsorb not only BMG but also microorganism components such as LPS and ET. These findings together with the ability of Lixelle to adsorb ET show the possibility of the application for treatment of endotoxinemia.
影响因子：
15.9
作者：
MARTIN, TR;MATHISON, JC;ULEVITCH, RJ
通讯作者：
ULEVITCH, RJ