TELOMERASE ACTIVITY IN NORMAL AND MALIGNANT HEMATOPOIETIC-CELLS
TELOMERASE ACTIVITY IN NORMAL AND MALIGNANT HEMATOPOIETIC-CELLS
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DOI：
10.1073/pnas.92.20.9082
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发表时间：
1995-09-26
影响因子：
11.1
通讯作者：
DELANGE, T
中科院分区：
文献类型：
--
作者：
BROCCOLI, D;YOUNG, JW;DELANGE, T
用聚合酶链反应（PCR）检测19例白血病患者的骨髓和外周血白细胞中端粒酶活性。端粒酶也可在正常供体的非恶性骨髓和外周血白细胞中检测到，包括富含粒细胞、T淋巴细胞和单核细胞/B细胞的组分。半定量比较显示正常人和白血病患者的样本中端粒酶活性之间存在相当大的重叠，混淆了端粒酶在这种疾病中的作用的评价。这些数据表明，人端粒酶不限于永生细胞，并表明这种酶的体细胞表达可能比以前从人端粒的下降推断的更广泛。
Bone marrow and peripheral blood leukocytes from 19 leukemia patients were found to contain telomerase activity detectable by a PCR-based assay. Telomerase was also detectable in nonmalignant bone marrow and peripheral blood leukocytes from normal donors, including fractions enriched for granulocytes, T lymphocytes, and monocytes/B cells. Semiquantitative comparison revealed considerable overlap between telomerase activities in samples from normal subjects and leukemia patients, confounding evaluation of the role of telomerase in this disease, These data indicate that human telomerase is not restricted to immortal cells and suggest that the somatic expression of this enzyme may be more widespread than was previously inferred from the decline of human telomeres.