CORRELATION OF ELECTROPHORETIC MOBILITIES OF PROTEINS AND PEPTIDES WITH THEIR PHYSICOCHEMICAL PROPERTIES
CORRELATION OF ELECTROPHORETIC MOBILITIES OF PROTEINS AND PEPTIDES WITH THEIR PHYSICOCHEMICAL PROPERTIES
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DOI：
10.1006/abio.1995.1190
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发表时间：
1995-03-20
影响因子：
2.9
通讯作者：
LADISCH, MR
中科院分区：
文献类型：
--
作者：
BASAK, SK;LADISCH, MR
9种蛋白质的电泳迁移率（μ）（M（r）14，200至70，000）在pH 8.77的28 mM Tris/47 mM甘氨酸缓冲液中的溶液和5 mM离子强度，并通过方程μ（f/f（0））=（Aq/M（r）（p）- B）与电荷（q）与分子量（M（r））和形状因子（f/f（0））的比率相关联。这种相关性先前报道的肽和蛋白质的μ在100 mM离子强度测量。当A = 6.048 × 10 ~（-3），B = 1.13 × 10 ~（-5），p = 2/3时，对于电泳迁移率在+13.35 × 10 ~（-5）~-19.7 × 10 ~（-5）cm ~（2）/（V.s）范围内的组分，在178 ~ 140，000的分子量范围内，该关联式拟合了51个实测值和文献值。实验测量证实了p = 2/3的普遍适用性，并表明电泳的常见电荷/质量关系适用于低离子强度缓冲液中的蛋白质，这是典型的电色谱系统。不同离子强度的相关性外推表明，低离子强度缓冲液放大了电泳迁移率的差异作为电荷/质量的函数，而高离子强度则减少了这种差异。(C)出版社：Academic Press
Electrophoretic mobilities, mu, of nine proteins (M(r) 14,200 to 70,000) in 28 mM Tris/47 mM glycine buffer at pH 8.77 and 5 mM ionic strength were measured by laser Doppler velocimetry and correlated to ratios of charge (q) to molecular weight (M(r)) and shape factor (f/f(0)) by the equation mu(f/f(0)) = (Aq/M(r)(p) - B). This correlation was previously reported for peptides and proteins for mu measured at 100 mM ionic strength. When A = 6.048 X 10(-3), B = 1.13 X 10(-5), and p = 2/3, the correlation fitted 51 measured and literature values over the molecular weight range of 178 to 140,000 for components whose electrophoretic mobilities ranged from +13.35 X 10(-5) to -19.7 X 10(-5) cm(2)/(V.s). The experimental measurements confirm the general suitability of p = 2/3 and show that the familiar charge/mass relation for electrophoresis is applicable to proteins in low-ionic-strength buffers which are typic al of electrochromatography systems. Extrapolation of the correlation to different ionic strengths indicates that a low-ionic-strength buffer amplifies differences of electrophoretic mobility as a function of charge/mass, while high ionic strength diminishes such differences. (C) 1995 Academic Press, Inc.