Cloning of an integrin beta subunit exhibiting high homology with integrin beta 3 subunit.
Cloning of an integrin beta subunit exhibiting high homology with integrin beta 3 subunit.
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与整合素β3亚基表现出高度同源性的整合素β亚基的克隆。
DOI：
10.1073/pnas.87.14.5354
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发表时间：
1990
影响因子：
11.1
通讯作者：
Tanihara,H
中科院分区：
文献类型：
--
作者：
Suzuki,S;Huang,ZS;Tanihara,H
利用聚合酶链式反应分离整合素家族的另一个β亚基的cDNA并进行测序。该序列全长3110个碱基对，有一个2388个碱基对的开放阅读框。推导的氨基酸序列与其他整合素β亚基相似，但不对应于β1、β2、β3或β4亚基。该β亚基被跨膜结构域划分为N-末端的大的胞外区和C-末端的小的胞内区。胞外区有一个富含半胱氨酸的区域，包含四个重复的8-半胱氨酸基序。在其他成熟的β亚基的胞外区中发现的所有56个半胱氨酸残基都存在于这个β亚基中。本文报道的β亚基与β3亚基具有特别高的同源性。该分子的mRNA大小约为3.5kBP，在各种细胞类型中都有表达。其他研究人员最近报告了与Vitronectin受体Alpha亚单位相关的额外的Beta亚基。该分子推导的氨基酸序列包含这些β亚基之一的N端部分氨基酸序列，βx。这里描述的β亚基似乎与βx亚基相同，并且作为玻璃连素受体的β亚基发挥作用。
cDNAs for another beta subunit of the integrin family were isolated with the aid of polymerase chain reaction and sequenced. The combined cDNA sequence is 3110 base pairs (bp) in size and has one long open reading frame of 2388 bp. The deduced amino acid sequence is similar to those of other integrin beta subunits but does not correspond to beta 1, beta 2, beta 3, or beta 4 subunits. This beta subunit is divided by a membrane-spanning domain into a large extracellular domain at the N-terminal side and a small intracellular domain at the C-terminal side. The extracellular domain has a cysteine-rich region that contains four repeats of 8-cysteine motifs. All 56 cysteine residues found in the extracellular domains of other mature beta subunits are present in this beta subunit. The beta subunit reported here has particularly high homology with the beta 3 subunit. The mRNA for the molecule is approximately 3.5 kbp in size and is expressed in various cell types. Other researchers have recently reported additional beta subunits that associate with the vitronectin receptor alpha subunit. The deduced amino acid sequence of this molecule contains the N-terminal partial amino acid sequence of one of these beta subunits, beta x. The beta subunit described herein seems to be identical to the beta x subunit and to function as the beta subunit of a vitronectin receptor.