Design of retroviral vectors for the insertion of foreign deoxyribonucleic acid sequences into the avian germ line.
Design of retroviral vectors for the insertion of foreign deoxyribonucleic acid sequences into the avian germ line.
复制标题
用于将外源脱氧核糖核酸序列插入禽种系的逆转录病毒载体的设计。
DOI：
10.3382/ps.0651459
复制
发表时间：
1986
期刊：
影响因子：
4.4
通讯作者：
L. Crittenden
中科院分区：
文献类型：
--
作者：
S. Hughes;E. Kosik;A. Fadly;D. Salter;L. Crittenden
由于现有的禽白血病病毒（ALV）载体在体内具有中等致癌性，因此它们不适合插入种系。通过用非致癌内源性 RAV-O 病毒的 LTR 取代 ALV 病毒的非编码长末端重复 (LTR) 区域，可以显着降低 ALV 载体的致癌性。有充分的证据表明，所得的 RAV-O LTR 载体可以插入家鸡的种系中，并且具有插入可用于家禽改良的克隆序列的潜力。
Because the available avian leukosis viral (ALV) vectors are moderately oncogenic in vivo, they are not suitable for insertion into the germ line. A significant reduction in the oncogenicity of the ALV vectors can be achieved by substituting the noncoding long terminal repeats (LTR) regions of the ALV virus with the LTR of the nononcogenic endogenous RAV-O virus. There is good evidence that the resulting RAV-O LTR vectors can be inserted into the germ line of domestic chickens and have the potential for inserting cloned sequences that can be used for poultry improvement.
DOI：
10.1073/pnas.77.12.7380
发表时间：
1980-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
GORDON, JW;SCANGOS, GA;RUDDLE, FH
通讯作者：
RUDDLE, FH