Intermediate and long-term exposure to air pollution and temperature and the extracellular microRNA profile of participants in the normative aging study (NAS).
Intermediate and long-term exposure to air pollution and temperature and the extracellular microRNA profile of participants in the normative aging study (NAS).
复制标题
规范衰老研究 (NAS) 参与者的中长期暴露于空气污染和温度以及细胞外 microRNA 谱。
DOI：
10.1016/j.envres.2023.115949
复制
发表时间：
2023
影响因子：
8.3
通讯作者：
Schwartz,JoelD
中科院分区：
文献类型：
--
作者：
DaneshYazdi,Mahdieh;Nassan,FeibyL;Kosheleva,Anna;Wang,Cuicui;Xu,Zongli;Di,Qian;Requia,WeeberbJ;Comfort,NicoleT;Wu,Haotian;Laurent,LouiseC;DeHoff,Peter;Vokonas,Pantel;Baccarelli,AndreaA;Schwartz,JoelD
研究背景中长期暴露于空气污染和温度的分子效应，例如细胞外microRNA上那些（ex-miRNA）还没有得到很好的理解，但可能有临床后果.ObjectivesTo评估暴露于环境空气污染和温度和ex-miRNA profiles.MethodsOur研究人群包括734名参与者在规范老化研究（NAS）之间的关联在1999年到2015年间。我们使用高分辨率模型来估计四周，八周，十二周，六个月和一年的PM2.5，O3，NO2和环境温度的移动平均值。感兴趣的结果是每个参与者随时间推移的细胞外microRNA（ex-miRNA）谱。我们使用纵向分位数回归方法来估计暴露与每个ex-miRNA之间的关联。结果进行了校正，多重比较和前仍然显着相关的暴露的miRNAs进行了进一步分析，使用KEGG通路分析和Incubity Pathway Analysis.ResultsWe发现151个显着的PM2.5，O3，NO2和环境温度和82个独特的前miRNAs在多个分位数的水平之间的关联。大多数重要结果与中期暴露于O3，长期暴露于PM2.5以及中期和长期暴露于环境温度有关。暴露最常与结局的第75和第90百分位数相关。重要的前-miRNA的通路分析揭示了他们参与的生物学途径，涉及细胞功能和通信以及临床疾病，如心血管疾病，呼吸系统疾病，和神经系统diseases.ConclusionOur的结果表明，中期和长期暴露于我们所有的利益暴露与前-miRNA的研究参与者的档案的变化。对环境风险因素和ex-miRNAs的进一步研究是必要的。
BackgroundThe molecular effects of intermediate and long-term exposure to air pollution and temperature, such as those on extracellular microRNA (ex-miRNA) are not well understood but may have clinical consequences.ObjectivesTo assess the association between exposure to ambient air pollution and temperature and ex-miRNA profiles.MethodsOur study population consisted of 734 participants in the Normative Aging Study (NAS) between 1999 and 2015. We used high-resolution models to estimate four-week, eight-week, twelve-week, six-month, and one-year moving averages of PM2.5, O3, NO2, and ambient temperature based on geo-coded residential addresses. The outcome of interest was the extracellular microRNA (ex-miRNA) profile of each participant over time. We used a longitudinal quantile regression approach to estimate the association between the exposures and each ex-miRNA. Results were corrected for multiple comparisons and ex-miRNAs that were still significantly associated with the exposures were further analyzed using KEGG pathway analysis and Ingenuity Pathway Analysis.ResultsWe found 151 significant associations between levels of PM2.5, O3, NO2, and ambient temperature and 82 unique ex-miRNAs across multiple quantiles. Most of the significant results were associations with intermediate-term exposure to O3, long-term exposure to PM2.5, and both intermediate and long-term exposure to ambient temperature. The exposures were most often associated with the 75th and 90th percentile of the outcomes. Pathway analyses of significant ex-miRNAs revealed their involvement in biological pathways involving cell function and communication as well as clinical diseases such as cardiovascular disease, respiratory disease, and neurological disease.ConclusionOur results show that intermediate and long-term exposure to all our exposures of interest were associated with changes in the ex-miRNA profile of study participants. Further studies on environmental risk factors and ex-miRNAs are warranted.