Surveillance of eight respiratory viruses in clinical samples of pediatric patients in Southeast Brazil
Surveillance of eight respiratory viruses in clinical samples of pediatric patients in Southeast Brazil
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DOI：
10.1590/s0021-75572007000600005
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发表时间：
2007-10-01
影响因子：
3.3
通讯作者：
Durigon, Edison L.
中科院分区：
文献类型：
--
作者：
Thomazelli, Luciano M.;Vieira, Sandra;Durigon, Edison L.
目的:检测的八个最常见呼吸道病毒:人类呼吸道合胞体病毒(HRSV)流感病毒A和B (IA和IB),副流感病毒病毒1、2和3 (HPIV1 2和3),腺病毒(广告)和人类metapneumovirus (HMPV),为了建立急性呼吸道感染(ARIs)的病原学和流行病学这些病毒的儿童在医院看到,大学大学de S (A) / tildeo保罗,在S (A) / tildeo圣保罗,巴西,在2003年。方法：对该院2003年1月1日至12月30日收治的所有5岁以下下呼吸道感染患儿进行流行病学监测。用聚合酶链反应对鼻和咽喉样本进行呼吸道病毒扫描，并采用GeneScan检测。结果：在336例患者的336份样本中，187例（55.6%）至少一种呼吸道病毒呈阳性。在所有儿童中，HRSV检出率为24.1%，HMPV检出率为17.8%，HPIV3检出率为8.3%，Ad检出率为6.8%，IA检出率为5%，HPIV1检出率为0.6%，44.1%的儿童未检出病毒。在所有样本中检测到双重病毒感染的占7.1%（阳性样本占12.8%）。本研究未检测到HPIV2和IB。结论：本研究证实5岁以下儿童特别是1岁以下儿童HRSV、HMPV、HPIV、流感和腺病毒的住院率较高。我们能够确定大多数急性呼吸道感染的病因学和流行病学，并追踪幼儿中最常见呼吸道病毒的季节性特征。
Objective: Detection of the eight most common respiratory viruses: human respiratory syncytial virus (HRSV), influenza virus A and B (IA and IB), parainfluenza viruses 1, 2 and 3 (HPIV1, 2 and 3), adenovirus (Ad) and human metapneumovirus (HMPV), in order to establish the etiology of acute respiratory infections (ARIs) and the epidemiology of these viruses in young children seen at Hospital Universitario, Universidade de S (a) over tildeo Paulo, in S (a) over tildeo Paulo, Brazil, during 2003.Methods: The epidemiological surveillance was conducted in all children younger than 5 years hospitalized at the Hospital for lower respiratory tract infections (LRTI) from January 1, 2003 to December 30, 2003. Nasal and throat samples were scanned for respiratory viruses by polymerase chain reaction and detected by the GeneScan assay.Results: Of 336 samples collected from 336 patients, 187 (55.6%) were positive for at least one of the respiratory viruses studied. Of all the children, HRSV was identified in 24.1%, HMPV in 17.8%, HPIV3 in 8.3%, Ad in 6.8%, IA in 5%, HPIV1 in 0.6%, but no virus could be detected in 44.1%. Dual virus infections were detected in 7.1% of all samples (12.8% of positive samples). HPIV2 and IB were not detected in the present study.Conclusions: This study confirms that children younger than 5 years and particularly younger than 1 year have a high hospitalization rate due to HRSV, HMPV, HPIV, influenza and adenovirus. We were able to determine the etiology and epidemiology of most ARIs and trace the seasonal profile of the commonest respiratory viruses among young children.