Stathmin expression modulates migratory properties of GN-11 neurons in vitro
Stathmin expression modulates migratory properties of GN-11 neurons in vitro
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DOI：
10.1210/en.2004-0972
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发表时间：
2005-04-01
期刊：
影响因子：
4.8
通讯作者：
Perroteau, I
中科院分区：
文献类型：
--
作者：
Giampietro, C;Luzzati, F;Perroteau, I
本实验研究了微管相关胞浆蛋白stathmin在体内GnRH神经内分泌系统中的表达，并对其功能进行了体外分析。在这里，我们提供了新的数据证明，在小鼠胚胎的鼻区和基底前脑区的GnRH迁移神经元表达stathmin蛋白。此外，这种表达模式依赖于位置，因为到达下丘脑的GnRH神经元是stathmin阴性的。永生化的GN-11细胞保留了许多GnRH神经元迁移的特征，强烈表达stathmin mRNA和蛋白。用GN-11细胞评价stathmin在GnRH迁移特性中的作用。在GN-11细胞中，我们上调(STMN)+和下调(STMN-)Stathmin的表达，并观察了细胞形态和运动能力的变化。过表达stathmin的细胞呈纺锤形，它们的增殖和运动能力比亲本细胞高。此外，与对照细胞相比，它们不聚集和表达低水平的钙粘附素。STMN-GN-11细胞具有多极突起，表现出运动性降低和高水平的钙粘蛋白表达。我们的发现表明，stathmin在GnRH细胞的运动中起着允许的作用，可能是通过调节钙粘附素的表达。
Expression of stathmin, a microtubule-associated cytoplasmic protein, prominently localized in neuroproliferative zones and neuronal migration pathways in brain, was investigated in the GnRH neuroendocrine system in vivo and the function was analyzed using an in vitro approach. Here we present novel data demonstrating that GnRH migrating neurons in nasal regions and basal forebrain areas of mouse embryos express stathmin protein. In addition, this expression pattern is dependent on location, as GnRH neurons reaching the hypothalamus are stathmin negative. Immortalized GN-11 cells, which retain many characteristics of migrating GnRH neurons, strongly express stathmin mRNA and protein. The role of stathmin in GnRH migratory properties was evaluated using GN-11 cell line. We up-regulated [ stathmin-transfected clones (STMN) +] and down-regulated ( STMN -) the expression of stathmin in GN-11 cells, and we investigated changes in cell morphology and motility in vitro. Cells overexpressing stathmin assume a spindle-shaped morphology and their proliferation, as well as their motility, is higher with respect to parental cells. Furthermore, they do not aggregate and express low levels of cadherins compared with control cells. STMN - GN-11 cells are endowed with multipolar processes, and they show a decreased motility and express high levels of cadherin protein. Our findings suggest that stathmin plays a permissive role in GnRH cell motility, possibly via modulation of cadherins expression.