Effects of Eucommia ulmoides Oliver leaf extract on 3T3-L1 differentiation into adipocytes
Effects of Eucommia ulmoides Oliver leaf extract on 3T3-L1 differentiation into adipocytes
复制标题
DOI：
10.1007/s11418-005-0026-y
复制
发表时间：
2006-02
影响因子：
3.3
通讯作者：
Eriko Matsuda;Y. Yoshizawa;Yukihito Yokosawa;N. Watanabe;S. Kawaii;N. Murofushi
中科院分区：
文献类型：
--
作者：
Eriko Matsuda;Y. Yoshizawa;Yukihito Yokosawa;N. Watanabe;S. Kawaii;N. Murofushi
本研究旨在探讨杜仲茶对小鼠3 T3-L1前脂肪细胞向脂肪细胞分化的影响。杜仲茶的沸水提取物抑制3 T3-L1细胞的脂质积累。提取物的HPLC分析鉴定了儿茶素、原儿茶酸、连苯三酚和绿原酸。儿茶素弱抑制3 T3-L1分化后的脂质积累，而原儿茶酸和绿原酸几乎没有效果。对杜仲茶进行活性导向分离，分离得到5-羟甲基糠醛（HMF）。HMF在100 μM浓度下抑制脂质蓄积，细胞中的脂质量降低至与阴性对照相似的水平。这是首次从杜仲茶中分离到HMF，并首次观察其对3 T3-L1分化的影响。
The extract prepared from roastedEucommia ulmoidesOliver leaves, Du-Zhong tea, was examined to explore its effect on differentiation of mouse 3T3-L1 preadipocyte cells into adipocytes. The boiling water extract of Du-Zhong tea inhibited lipid accumulation in 3T3-L1. The HPLC analysis of the extract identified catechin, protocatechuic acid, pyrogallol, and chlorogenic acid. Catechin weakly inhibited lipid accumulation after 3T3-L1 differentiation, while protocatechuic acid and chlorogenic acid showed almost no effect. The activity guided separation of Du-Zhong tea lead the isolation of 5-hydroxymethyl-2-furaldehyde (HMF). HMF inhibited lipid accumulation at a concentration of 100 μM, and the amount of lipid in the cells was reduced to the similar level of negative control. This is the first isolation of HMF from Du-Zhong tea and the first observation of its effect on 3T3-L1 differentiation.