TRANSFORMATION AND RESTORATION OF BICONCAVE SHAPE OF HUMAN ERYTHROCYTES INDUCED BY AMPHIPHILIC AGENTS AND CHANGES OF IONIC ENVIRONMENT
TRANSFORMATION AND RESTORATION OF BICONCAVE SHAPE OF HUMAN ERYTHROCYTES INDUCED BY AMPHIPHILIC AGENTS AND CHANGES OF IONIC ENVIRONMENT
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DOI：
10.1016/0005-2736(68)90078-3
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发表时间：
1968-01-01
期刊：
影响因子：
--
通讯作者：
DEUTICKE, B
中科院分区：
文献类型：
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作者：
DEUTICKE, B
1.1.正常人红细胞的双凹面盘状形状可以被大量的两亲性试剂可逆地改变。锯齿状细胞通常由阴离子或非离子化合物诱导;杯状细胞由阳离子物质诱导。锯齿状细胞和杯状细胞的形成可能是由转化剂与膜内带相反电荷的位点相互作用诱导的。诱导锯齿状或杯状形成的化合物可以被认为是真正的拮抗剂，因为任何一组化合物都能使另一组化合物诱导的形状转变完全逆转。将红细胞悬浮在非渗透性阴离子的等渗溶液中会导致杯细胞的发生，随着细胞外pH值的降低，转化的程度变得更加明显。在这些条件下，通过加入渗透性阴离子来恢复正常形状。产生90%正常化所需的这些阴离子的浓度随着pH值的下降而增加。这种类型的杯状形成据推测是由红细胞膜和相邻介质之间的pH值差的临界值以上的增加引起的。结果表明，红细胞膜的结构元素之间的相互作用具有很大的不稳定性，红细胞膜的结构元素负责维持双凹形状。他们认为这种形状是由分别指向圆齿细胞或杯状细胞形成的相反力量之间的平衡造成的。
1.1. The biconcave discoid shape of normal human erythrocytes can be altered reversibly by a great number of amphiphilic agents. Crenated cells are induced, as a rule, by anionic or non-ionized compounds; cup-like cells by cationic substances. Formation of crenated and cup cells probably is induced by interaction of the transforming agents with sites charged oppositely within the membrane.2.2. Compounds inducing crenation or cup formation can be regarded as true antagonists, since either group of compounds regularily brings about complete reversal of shape transformatins induced by the other one.3.3. Suspending erythrocytes in isotonic solutions of non-penetrating anions leads to the occurrence of cup cells, the extent of transformation becoming more pronounced with lowering of extracellular pH. Restoration of normal shape under these conditions is accomplished by addition of penetrating anions. The concentrations of these anions necessary to produce 90% normalisation increase with fall of pH. This type of cup formation supposedly is induced by the increase above a critical value of the pH difference between erythrocyte membrane and adjacent media.4.4. The results point to a great lability of the interactions of structural elements of the erythrocyte membrane responsible for the maintenance of biconcave shape. They suggest this shape to result from an equilibrium between opposite forces directed towards formation of crenated or cup cells, respectively.