Cascade regulation of spore coat gene expression in Bacillus subtilis.
Cascade regulation of spore coat gene expression in Bacillus subtilis.
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DOI：
10.1016/0022-2836(90)90227-d
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发表时间：
1990-04
影响因子：
5.6
通讯作者：
Liangbiao Zheng;R. Losick
中科院分区：
文献类型：
--
作者：
Liangbiao Zheng;R. Losick
Endospores of the Gram-positive bacteriumBacillus subtilisare encased in a tough protein shell known as the coat. The coat is composed of a dozen or more different structural proteins. We report the identification of and studies on the regulation of promoters governing the expression of coat protein (cot) genes designatedBtoEencoding polypeptides of 59, 12, 11 and 24 kDa, respectively. We show that transcription of genesB,CandDis governed by single promoters and that transcription of geneEis governed by tandem promoters designated P1 and P2. In extension of recent work on the transcription ofcotgeneAand the mother-cell regulatory genesgerE,sigKandspoIIID, we show that genes involved in coat formation are turned on in a regulatory cascade of at least four co-ordinately controlled gene sets. The cascade consists of:cotEas transcribed from its P1 promoter andspoIIID, which are turned on during hours three to four of sporulation;cotEas transcribed from its P2 promoter andsigK, which are turned on during hour five by the appearance of the product (a small DNA-binding protein) ofspoIIID;cotA,cotDandgerE, which are turned on during hours five to six by the appearance of the product (sigma factorσK) ofsigK; andcotBandcotC, which are turned on during hour seven by the appearance of the product (an inferred DNA-binding protein) ofgerE. The cascade is hierarchical in that the first three gene sets each contain the regulatory gene that turns on the expression of the next gene set in the pathway. We also show that the level of expression of a member (cotC) of the terminal class of gene expression is strongly influenced by medium and that this effect directly or indirectly depends on the product of sporulation genespoIVA.
