Counting cells with the new stereology.
Counting cells with the new stereology.
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DOI：
10.1016/0962-8924(92)90038-o
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发表时间：
1992-06-01
影响因子：
19
通讯作者：
Nurcombe, V
中科院分区：
文献类型：
--
作者：
Bertram, J F;Nurcombe, V
也许在以前的许多细胞数量报告中，更大的不足是细胞数量相对于单位组织体积(细胞数密度)的表达。现已确定，组织和器官的组成部分在为显微镜检查准备组织的不同溶剂中会有不同程度的收缩和膨胀4，S。不能假设器官的不同组成部分收缩或膨胀到相同的程度；也不能假设发育中、老化或患病的组织会经历与正常组织相同的体积变化。因此，细胞数密度可以增加，而器官中的细胞总数保持不变，甚至可能减少！在这种情况下，细胞数密度比实际细胞数更能说明差异收缩。
Perhaps an even greater deficiency in many previous reports of cell numbers is the expression of cell number relative to a unit volume of tissue (cell numerical density). It is now well established that components of tissues and organs shrink and swell to differing extents in the various solvents used to prepare tissue for microscopic examination4, s. It cannot be assumed that different components of an organ shrink or swell to the same extent; nor can it be assumed that developing, aged or diseased tissues will undergo the same volumetric changes as normal tissues. Therefore, cell numerical density can increase while the total number of cells in an organ stays the same or possibly even decreases! In this case, ceil numerical density tells us more about differential shrinkage than actual cell number.