General Regulatory Factors (GRFs) as genome partitioners
General Regulatory Factors (GRFs) as genome partitioners
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DOI：
10.1074/jbc.m202578200
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发表时间：
2002-11-01
影响因子：
4.8
通讯作者：
Gilson, É
中科院分区：
文献类型：
--
作者：
Fourel, G;Miyake, T;Gilson, É
绝缘子是将相邻的染色体区域解偶联的序列。在这里，我们已经证明了酿酒酵母Rap1p和Abf1p蛋白具有强大的绝缘能力。Rap1p中的绝缘结构域与先前描述的转录激活结构域一致，而跨越Abf1p C末端(440-731)的四个相邻亚结构域被发现显示出自主绝缘能力。Rap1p和Abf1p沉默结构域都包含或在很大程度上与绝缘结构域重叠，这表明绝缘传递了一些未定义的染色体组织能力，这也在沉默中起到了作用。与以前参与酿酒酵母亚端粒绝缘子活性的Reb1p和Tbf1p一起，绝缘电位作为一般调节因子(GRF)的补充共同属性出现。因此，GRFs不仅在调节基因表达方面发挥关键作用，而且还可以将基因组划分为功能独立的区域。
Insulators are sequences that uncouple adjacent chromosome domains. Here we have shown that Saccharomyces cerevisiae Rap1p and Abf1p proteins are endowed with a potent insulating capacity. Insulating domains in Rap1p coincide with previously described transcription activation domains, whereas four adjacent subdomains spanning the whole of the Abf1p C terminus (440-731) were found to display autonomous insulating capacity. That both Rap1p and Abf1p silencing domains either contain or largely overlap with an insulating domain suggests that insulation conveys some undefined chromosome organization capacity that also contributes a function in silencing. Together with Reb1p and Tbf1p, previously involved in the activity of Saccharomyces cerevisiae subtelomeric insulators, insulating potential emerges as a supplementary common property of General Regulatory Factors (GRFs). Thus GRFs, which bind to sites scattered throughout the genome within promoters, would not only play a key role in regulating gene expression but also partition the genome in functionally independent domains.