Inhibition of swarming motility of Pseudomonas aeruginosa by branched-chain fatty acids
Inhibition of swarming motility of Pseudomonas aeruginosa by branched-chain fatty acids
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DOI：
10.1111/j.1574-6968.2008.01089.x
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发表时间：
2008-04-01
影响因子：
2.1
通讯作者：
Fukui, Kazuhiro
中科院分区：
文献类型：
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作者：
Inoue, Tetsuyoshi;Shingaki, Ryuji;Fukui, Kazuhiro
铜绿假单胞菌能够通过游泳、蜂群和抽搐运动来移动。在本研究中，我们研究了脂肪酸对铜绿假单胞菌PAO1活性的影响。支链脂肪酸(BCFA) - 12-甲基十四烷酸(anteiso-C15:0) -在浓度为10 μ g mL（-1）时，可以轻微抑制鞭毛驱动的游动运动，并完全抑制更复杂的表面运动，即群体运动。相反，anteiso-C15:0对瞳孔抽搐运动没有影响。本研究中测试的其他BCFAs和不饱和脂肪酸对蜂群运动也有类似的抑制作用，尽管这些脂肪酸的抑制水平不同。这些脂肪酸在液体培养中没有明显的生长抑制作用。直链饱和脂肪酸如棕榈酸对蜂群的抑制效果较差。添加anteiso-C15:0后，PAO1菌落的湿度显著降低；然而，作为表面活性剂的鼠李糖脂的生产不受脂肪酸的影响。除了抑制运动外，anteiso-C15:0还能抑制PAO1形成31%的生物膜，这表明BCFA可能影响铜绿假单胞菌的多种细胞活性。
Pseudomonas aeruginosa is capable of moving by swimming, swarming, and twitching motilities. In this study, we investigated the effects of fatty acids on Pseudomonas aeruginosa PAO1 motilities. A branched-chain fatty acid (BCFA) - 12-methyltetradecanoic acid (anteiso-C15:0) - has slightly repressed flagella-driven swimming motility and completely inhibited a more complex type of surface motility, i.e. swarming, at a concentration of 10 mu g mL(-1). In contrast, anteiso-C15:0 exhibited no effect on pili-mediated twitching motility. Other BCFAs and unsaturated fatty acids tested in this study showed similar inhibitory effects on swarming motility, although the level of inhibition differed between these fatty acids. These fatty acids caused no significant growth inhibition in liquid cultures. Straight-chain saturated fatty acids such as palmitic acid were less effective in swarming inhibition. The wetness of the PAO1 colony was significantly reduced by the addition of anteiso-C15:0; however, the production of rhamnolipids as a surface-active agent was not affected by the fatty acid. In addition to motility repression, anteiso-C15:0 caused 31% repression of biofilm formation by PAO1, suggesting that BCFA could affect the multiple cellular activities of Pseudomonas aeruginosa.