A new member of a hepatoma-derived growth factor gene family can translocate to the nucleus
A new member of a hepatoma-derived growth factor gene family can translocate to the nucleus
复制标题
DOI：
10.1006/bbrc.1999.1733
复制
发表时间：
1999-12-09
影响因子：
3.1
通讯作者：
Nakamura, H
中科院分区：
文献类型：
--
作者：
Ikegame, K;Yamamoto, M;Nakamura, H
肝癌源性生长因子 (HDGF) 和 HDGF 相关蛋白 (HRP) 属于 N 末端（hath 区域）具有高度保守氨基酸序列的基因家族。人类和小鼠 HDGF 家族的新成员被鉴定并克隆；我们称之为 HRP-3。从 HRP-3 cDNA 推导的氨基酸序列包含 203 个氨基酸，不含分泌信号肽。 HRP-3 N 末端有 97 个氨基酸序列，与 HDGF 家族蛋白的 hath 区域高度保守。它还在其 HDGF 和 HRP-1 自身特异性区域的相似位置具有推定的二分核定位信号 (NLS) 序列。 Northern印迹分析表明，HRP-3主要在人类的睾丸和大脑中表达，在心脏中有中等程度的表达，在卵巢、肾脏、脾脏和肝脏中也有少量表达。转染绿色荧光蛋白（GFP）标记的HRP-3 cDNA表明HRP-S易位至293细胞的细胞核。 GFP-HRP-3 转染子比仅用载体转染的细胞显着增加了 DNA 合成。通过 FISH 分析将 HRP-3 基因定位到 15 号染色体 q25 区域。这些发现表明，HDGF 基因家族的新成员 HRP-3 可能主要在大脑、睾丸和心脏的细胞核中发挥作用，可能与细胞增殖有关。 (C) 1999 年学术出版社。
Hepatoma-derived growth factor (HDGF) and HDGF-related proteins (HRP) belong to a gene family with a well-conserved amino acid sequence at the N-terminus (the hath region). A new member of the HDGF family in humans and mice was identified and cloned; we call it HRP-3. The deduced amino acid sequence from HRP-3 cDNA contained 203 amino acids without a signal peptide for secretion. HRP-3 has its 97-amino-acid sequence at the N-terminus, which is highly conserved with the hath region of the HDGF family proteins. It also has a putative bipartite nuclear localizing signal (NLS) sequence in a similar location in its self-specific region of HDGF and HRP-1. Northern blot analysis shows that HRP-3 is expressed predominantly in the testis and brain, to an intermediate extent in the heart, and to a slight extent in the ovaries, kidneys, spleen, and liver in humans. Transfection of green fluorescent protein (GFP)-tagged HRP-3 cDNA showed that HRP-S translocated to the nucleus of 293 cells. GFP-HRP-3 transfectants significantly increased their DNA synthesis more than cells transfected with vector only. The HRP-3 gene was mapped to chromosome 15, region q25 by FISH analysis. These findings suggest that a new member of the HDGF gene family, HRP-3, may function mainly in the nucleus of the brain, testis, and heart, probably for cell proliferation. (C) 1999 Academic Press.