Identification of Neuronal Lineages in the Drosophila Peripheral Nervous System with a "Digital" Multi-spectral Lineage Tracing System.
Identification of Neuronal Lineages in the Drosophila Peripheral Nervous System with a "Digital" Multi-spectral Lineage Tracing System.
复制标题
用“数字”多光谱谱系追踪系统鉴定果蝇周围神经系统中的神经元谱系。
DOI：
10.1016/j.celrep.2019.10.124
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发表时间：
2019
期刊：
影响因子：
8.8
通讯作者：
Cai,Dawen
中科院分区：
文献类型：
--
作者：
Veling,MacyW;Li,Ye;Veling,MikeT;Litts,Christopher;Michki,Nigel;Liu,Hao;Ye,Bing;Cai,Dawen
阐明细胞谱系有助于对发育的重要理解。最近发展起来的基于测序的技术提高了谱系追踪的规模，但消除了传统方法提供的空间信息。多光谱标记技术，如脑弓，有可能在原位鉴定与血统相关的细胞。在这里，我们报告了核比特弓(NBitbown)，这是一种“数字”版本的脑弓，它极大地扩展了评分细胞的颜色多样性，以及一套用于量化任意两个细胞的谱系关系的统计方法。将这些工具应用于果蝇外周神经系统(PNS)，我们确定了所有神经元对之间的谱系关系。这项研究表明nBitrow是一种有效的定位工具，而幼年三叉神经节神经元之间的完整谱系关系为研究神经元如何获得特定功能和电路连接打开了新的可能性。
Elucidating cell lineages provides crucial understanding of development. Recently developed sequencing-based techniques enhance the scale of lineage tracing but eliminate the spatial information offered by conventional approaches. Multi-spectral labeling techniques, such as Brainbow, have the potential to identify lineage-related cellsin situ. Here, we report nuclear Bitbow (nBitbow), a "digital" version of Brainbow that greatly expands the color diversity for scoring cells, and a suite of statistical methods for quantifying the lineage relationship of any two cells. Applying these tools to theDrosophilaperipheral nervous system (PNS), we determined lineage relationship between all neuronal pairs. This study demonstrates nBitbow as an efficient tool forin situlineage mapping, and the complete lineage relationship among larval PNS neurons opens new possibilities for studying how neurons gain specific features and circuit connectivity.