Number and Replating Capacity of Endothelial Colony-Forming Cells are Telomere Length Dependent: Implication for Human Atherogenesis.
Number and Replating Capacity of Endothelial Colony-Forming Cells are Telomere Length Dependent: Implication for Human Atherogenesis.
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内皮集落形成细胞的数量和再生能力取决于端粒长度：对人类动脉粥样硬化的影响。
DOI：
10.1161/jaha.120.020606
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发表时间：
2021-05-18
影响因子：
5.4
通讯作者：
TELARTA consortium †
中科院分区：
文献类型：
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作者：
Toupance S;Simonici S;Labat C;Dumoulin C;Lai TP;Lakomy C;Regnault V;Lacolley P;Dignat George F;Sabatier F;Aviv A;Benetos A;TELARTA consortium †
白细胞端粒长度短（TL）与动脉粥样硬化性心血管疾病有关。内皮修复在动脉粥样硬化的发展中起着关键作用。目的是研究TL与内皮集落形成细胞（ecfc）增殖动力学之间的关系，ecfc作为祖细胞表现出内皮修复活性。为了分离ecfc，我们在TELARTA（动脉老化端粒）队列研究中对116名参与者（24-94岁）的血液样本进行了克隆测定。我们在29个（第1组）中未检测到ECFC克隆，在另外29个（第2组）中检测到没有复制能力的克隆，在另外58个（第3组）中检测到有复制能力的克隆。白细胞TL采用Southern blotting和ECFC （ECFC‐TL）测定。年龄和性别调节的白细胞TL（平均±SEM）以组1最短（6.51±0.13 kb），组2较长（6.69±0.13 kb），组3最长（6.78±0.09 kb） （P<0.05）。在第3组，ECFC‐TL与检测到的克隆数相关（P<0.01）。ECFC‐TL（7.98±0.13 kb）比白细胞TL（6.74±0.012 kb）长（P<0.0001），两者呈强相关（r=0.82； P<0.0001）。端粒较长的个体显示出更多的自我更新的ecfc。我们的研究结果还表明，白细胞TL作为ecfc中TL动态的代表，可以作为临床和实验室实践中内皮修复能力的替代标志物，因为白细胞很容易获得。URL: https://www.clinicaltrials.gov;唯一标识符：NCT02176941。
Short leukocyte telomere length (TL) is associated with atherosclerotic cardiovascular disease. Endothelial repair plays a key role in the development of atherosclerosis. The objective was to examine associations between TL and proliferative dynamics of endothelial colony‐forming cells (ECFCs), which behave as progenitor cells displaying endothelial repair activity. To isolate ECFCs, we performed a clonogenic assay on blood samples from 116 participants (aged 24–94 years) in the TELARTA (Telomere in Arterial Aging) cohort study. We detected no ECFC clones in 29 (group 1), clones with no replating capacity in other 29 (group 2), and clones with replating capacity in the additional 58 (group 3). Leukocyte TL was measured by Southern blotting and ECFCs (ECFC‐TL). Age‐ and sex‐adjusted leukocyte TL (mean±SEM) was the shortest in group 1 (6.51±0.13 kb), longer in group 2 (6.69±0.13 kb), and the longest in group 3 (6.78±0.09 kb) (P<0.05). In group 3, ECFC‐TL was associated with the number of detected clones (P<0.01). ECFC‐TL (7.98±0.13 kb) was longer than leukocyte TL (6.74±0.012 kb) (P<0.0001) and both parameters were strongly correlated (r=0.82; P<0.0001). Individuals with longer telomeres display a higher number of self‐renewing ECFCs. Our results also indicate that leukocyte TL, as a proxy of TL dynamics in ECFCs, could be used as a surrogate marker of endothelial repair capacity in clinical and laboratory practice because of easy accessibility of leukocytes. URL: https://www.clinicaltrials.gov; Unique identifier: NCT02176941.