Changes in Cell Size and Flagellation in the Phytopathogen Pseudomonas syringae pv. tabaci Cultured in vitro and in planta: A Comparative Electron Microscope Study
Changes in Cell Size and Flagellation in the Phytopathogen Pseudomonas syringae pv. tabaci Cultured in vitro and in planta: A Comparative Electron Microscope Study
复制标题
植物病原体丁香假单胞菌细胞大小和鞭毛的变化。
DOI：
10.1111/j.1439-0434.1989.tb01063.x
复制
发表时间：
1989
影响因子：
1.5
通讯作者：
M. Elmasry
中科院分区：
文献类型：
--
作者：
D. Sigee;M. Elmasry
在平行实验中，假单胞菌pv.将烟粉虱渗透到烟草叶中（以确定植物中发生的细菌变化）并接种到营养肉汤中（对体外培养的细胞进行比较观察）。在每种情况下，表面结构，细菌大小和鞭毛的细节，确定在一系列的样品通过透射电子显微镜的整个安装染色和未染色的准备。
在植物和体外环境中，细菌种群均表现出明显的指数增长阶段。在每种情况下，细菌的大小是最高的增殖阶段的早期部分，然后在其余的增殖期下降。在每种情况下，具有鞭毛的细胞的比例也显示出类似的趋势-在浸润后最初减少，然后在细菌增殖阶段主要增加。这些结果表明，在植物中的细菌大小和鞭毛的变化直接关系到人口的增长阶段，因此，主要是由内部细胞（内源性）因素，而不是由外部因素在叶环境。
In parallel experiments, cells of Pseudomonas syringae pv. tabaci were infiltrated into tobacco leaves (to determine bacterial changes occurring in planta) and inoculated into nutrient broth (to make comparative observations on cells cultured in vitro). In each case, details of surface structure, bacterial size and flagellation were determined in a sequence of samples by transmission electron microscopy of whole mount stained and unstained preparations.
In both in planta and in vitro environments, bacterial population showed a clear phase of exponential increase. In each case, bacterial size was highest during the early part of the multiplication phase, then decreased during the rest of the multiplication period. In each case also, the proportion of cells with flagella showed a similar trend - with an initial decrease after infiltration, followed by a major increase during the phase of bacterial multiplication. These results suggest that changes in bacterial size and flagellation in planta relate directly to the growth phase of the population, and are therefore determined primarily by internal cellular (endogenous) factors - rather than by external factors within the leaf environment.