Effects of thiol protease inhibitors on intracellular degradation of exogenous beta-galactosidase in cultured human skin fibroblasts.
Effects of thiol protease inhibitors on intracellular degradation of exogenous beta-galactosidase in cultured human skin fibroblasts.
复制标题
硫醇蛋白酶抑制剂对外源性β-半乳糖苷酶在培养的人皮肤成纤维细胞内降解的影响。
DOI：
10.1016/0014-4827(83)90175-1
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发表时间：
1983
影响因子：
3.7
通讯作者：
Y. Suzuki
中科院分区：
文献类型：
--
作者：
Y. Ko;T. Yamanaka;M. Umeda;Y. Suzuki
本文研究了微生物来源的低分子量蛋白酶抑制剂对β-半乳糖苷酶缺乏的人皮肤成纤维细胞胞内β-半乳糖苷酶降解的影响。只有巯基蛋白酶抑制剂显示出增加酶活性的效果。巯基蛋白酶的特异性抑制剂E-64使外源性β-半乳糖苷酶的半衰期延长3倍，当酶以脂质体形式提供时，在这些细胞中的半衰期延长9倍。讨论了巯基蛋白酶在酶分子降解中的作用。
The effects of low molecular weight (LMW) protease inhibitors of microbial origin were evaluated on the intracellular degradation of β-galactosidase purified fromAspergillus oryzaeand taken up by cultured human skin fibroblasts with β-galactosidase deficiency. Only thiol protease inhibitors showed an effect to increase the enzyme activity. E-64, a specific inhibitor of thiol proteases, prolonged 3-fold a half life of the exogenous β-galactosidase and when the enzyme was supplied as liposomes, the half life was prolonged 9-fold in these cells. The role of thiol proteases in the degradation of enzyme molecules was discussed.