The diagnostic and prognostic utility of claudin expression in renal cell neoplasms
The diagnostic and prognostic utility of claudin expression in renal cell neoplasms
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DOI：
10.1038/modpathol.2008.116
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发表时间：
2008-11-01
期刊：
影响因子：
7.5
通讯作者：
Wang, Li J.
中科院分区：
文献类型：
--
作者：
Lechpammer, Mirna;Resnick, Murray B.;Wang, Li J.
本研究评估了密蛋白 1、3、4、7 和 8 在人肾细胞癌和嗜酸细胞瘤中的表达模式，并将表达与患者预后相关。利用来自 141 名肾细胞癌或嗜酸细胞瘤患者的石蜡包埋组织样本（90 名透明细胞癌、22 名乳头状癌、17 名嫌色肾细胞癌和 12 名嗜酸细胞瘤）制作组织微阵列。密蛋白 3、4、7 和 8 的染色模式是膜性和/或细胞质的，而密蛋白 1 在非肿瘤性肾组织和肿瘤中主要是膜性的。 Fuhrman 1 级和 2 级透明细胞肾细胞癌中主要检测到阴性或弱密蛋白 3 染色（78%；P = 0.016），这表明密蛋白 3 的上调可能与透明细胞肾细胞癌级别的增加同时发生。此外，Kaplan-Meier 单变量分析显示，中度至强密蛋白 3 和 4 表达与透明细胞肾细胞癌的总生存率呈显着负相关（P = 0.038 和 P = 0.031）。中度至强的紧密连接蛋白 7 表达在嫌色肾细胞癌 (94%) 中比在嗜酸细胞瘤 (55%；P = 0.041) 中更为常见。 92% 的嗜酸细胞瘤的 Claudin 8 染色呈中等到强，这将它们与乳头状肾细胞癌和透明细胞肾细胞癌区分开来（14% 和 12%；均为 P
This study evaluated the expression patterns of claudins 1, 3, 4, 7, and 8 in human renal cell carcinomas and oncocytomas and correlated expression with patient prognosis. Tissue microarrays were created from paraffin-embedded tissue samples from 141 patients with renal cell carcinomas or oncocytoma (90 clear cell, 22 papillary, 17 chromophobe renal cell carcinomas, and 12 oncocytomas). The staining pattern for claudins 3, 4, 7, and 8 was membranous and/or cytoplasmic, whereas claudin 1 was predominantly membranous in both nonneoplastic renal tissue and tumors. Negative to weak claudin 3 staining was predominantly detected in Fuhrman's grade 1 and 2 clear cell renal cell carcinomas (78%; P = 0.016), suggesting that upregulation of claudin 3 potentially occurs concomitantly with increasing grade of clear cell renal cell carcinomas. In addition, Kaplan-Meier univariate analysis showed a significant inverse correlation between moderate to strong claudin 3 and 4 expression with overall survival in clear cell renal cell carcinomas (P = 0.038 and P = 0.031). Moderate to strong claudin 7 expression was significantly more common in chromophobe renal cell carcinomas (94%) than in oncocytomas (55%; P = 0.041). Claudin 8 staining was moderate to strong in 92% of oncocytomas, which differentiated them from papillary and clear cell renal cell carcinomas (14 and 12%; both P