High-throughput proteomics: a methodological mini-review.
High-throughput proteomics: a methodological mini-review.
复制标题
DOI：
10.1038/s41374-022-00830-7
复制
发表时间：
2022-11
影响因子：
5
通讯作者：
Zhang, Lanjing
中科院分区：
文献类型：
--
作者：
Cui, Miao;Cheng, Chao;Zhang, Lanjing
蛋白质组学在后基因组时代的生物医学研究中起着至关重要的作用。随着技术革命和新兴的计算和统计模型，蛋白质组学方法在过去的十年中迅速发展，并为解决复杂的生物医学问题提供了光明。本文总结了现有和新兴的高通量蛋白质组学方法的科学研究和临床实践，包括质谱、蛋白质通路阵列、新一代组织微阵列、单细胞蛋白质组学、单分子蛋白质组学、Luminex、Simoa和Olink蛋白质组学。我们还讨论了重要的计算方法和统计算法，可以最大限度地挖掘蛋白质组数据与临床和/或其他'组学数据。为了更好地利用这些工具，提供了各种原则和预防措施。总之，高通量蛋白质组学的进展不仅有助于更好地了解发病机制的分子机制，而且还有助于识别特定疾病的信号网络。因此，现代蛋白质组学在基础研究、预后肿瘤学、精准医学和药物发现方面具有一系列潜在的应用。蛋白质组学在后基因组时代的生物医学研究中起着至关重要的作用。随着技术革命和计算工具的出现，蛋白质组学方法在过去十年中得到了迅速发展，并为解决复杂的生物医学问题提供了光明。因此，本文简要综述了现有和新兴的高通量蛋白质组学方法，包括质谱、蛋白质通路阵列、下一代组织微阵列、单细胞蛋白质组学、单分子蛋白质组学、Luminex、Simoa和OLINK蛋白质组学。
Proteomics plays a vital role in biomedical research in the post-genomic era. With the technological revolution and emerging computational and statistic models, proteomic methodology has evolved rapidly in the past decade and shed light on solving complicated biomedical problems. Here, we summarize scientific research and clinical practice of existing and emerging high-throughput proteomics approaches, including mass spectrometry, protein pathway array, next-generation tissue microarrays, single-cell proteomics, single-molecule proteomics, Luminex, Simoa and Olink Proteomics. We also discuss important computational methods and statistical algorithms that can maximize the mining of proteomic data with clinical and/or other ‘omics data. Various principles and precautions are provided for better utilization of these tools. In summary, the advances in high-throughput proteomics will not only help better understand the molecular mechanisms of pathogenesis, but also to identify the signature signaling networks of specific diseases. Thus, modern proteomics have a range of potential applications in basic research, prognostic oncology, precision medicine, and drug discovery. Proteomics plays a vital role in biomedical research in the post-genomic era. With the technological revolution and emerging computational tools, proteomic methodology has evolved rapidly in the past decade and shed light on solving complicated biomedical problems. Thus, this mini-review summarizes existing and emerging high-throughput proteomics methodologies, including mass spectrometry, protein pathway array, next-generation tissue microarrays, single-cell proteomics, single-molecule proteomics, Luminex, Simoa and OLINK Proteomics.
影响因子：
3.6
作者：
Zysset D;Montani M;Spalinger J;Schibli S;Zlobec I;Mueller C;Sokollik C
通讯作者：
Sokollik C
影响因子：
3.7
作者：
Buhimschi, Irina A.;Zhao, Guomao;Rosenberg, Victor A.;Abdel-Razeq, Sonya;Thung, Stephen;Buhimschi, Catalin S.
通讯作者：
Buhimschi, Catalin S.