Toward the development of an infectious cDNA clone of a human enteric coronavirus.
Toward the development of an infectious cDNA clone of a human enteric coronavirus.
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DOI：
10.1007/978-0-387-33012-9_95
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发表时间：
2006
影响因子：
--
通讯作者：
Zhang X
中科院分区：
文献类型：
--
作者：
Zhu H;Liu Y;Cai Y;Yu D;Pu Y;Harmon L;Zhang X
1. INTRODUCTION respiratory, digestive, neurological, and immune-mediated diseases in human and animals. The outbreak of the severe acute respiratory syndrome (SARS) in 2003 was caused by SARS-CoV, which is most closely related to group II CoV, especially the bovine CoV (BCoV). Although the exact origin of SARS-CoV remains to be identified, the fact that similar SARS-CoV has been repeatedly isolated in wild animals, including civet cat, raccoon dog, and badges, strongly suggests that SARS-CoV originates from animals. It was postulated that SARS-CoV is a zoonotic pathogen. 1 Ironically, the zoonotic nature of CoV had been documented long before the outbreak of SARS. In 1994, Zhang et al., 2 reported the isolation of a human enteric CoV (HECoV) from a 6-year old child with partial sequence of the structural genes revealed that HECoV is more closely related to BCoV than to other members of the CoV family. Interestingly, several studies showed that BCoV was able to infect other small ruminants, turkey, and dogs and cause clinical diseases in these animals, thus further supporting the idea that BCoV is a zoonotic pathogen. Therefore, BCoV is the first documented CoV that can cross species barrier and infect animals ranging from avian, ruminant, carnivore, to humans. In the present study, we attempted to develop an infectious cDNA clone of HECoV by reverse genetics, which will provide a powerful genetic tool for future studies of the molecular evolution and animal to human transmission, and the molecular pathogenesis, and for the design of preventive and therapeutic interventions.
