Regeneration and tolerance factor modulates the effect of adenosine triphosphate-induced interleukin 1β secretion in human macrophages
Regeneration and tolerance factor modulates the effect of adenosine triphosphate-induced interleukin 1β secretion in human macrophages
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DOI：
10.1016/j.humimm.2004.04.006
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发表时间：
2004-07-01
期刊：
影响因子：
2.7
通讯作者：
Beaman, K
中科院分区：
文献类型：
--
作者：
Derks, R;Beaman, K
这些研究描述了一种称为再生和耐受因子（RTF）的分子，它通过调节白细胞介素1 β（IL-1 β）的分泌来控制炎症。最近，已经证明三磷酸腺苷（ATP）与P2 X7嘌呤受体的相互作用诱导IL-1 β的分泌并启动炎症反应。在这些实验中，发现向巨噬细胞中添加ATP诱导P2 X7活化和IL-1 β的分泌。这种分泌用抗RTF抗体与外源性ATP的组合增强（p< 0.005）。RTF还显示能够影响表面ATP酶活性，并增加PI掺入，这是P2 X7活化的指标。我们证明了RTF通过调节P2 X7活性在控制IL-1 β分泌中起作用。
These studies characterize a molecule known as regeneration and tolerance factor (RTF), which controls inflammation by regulating interleukin 1beta (IL-1beta) secretion. Recently, it has been demonstrated that the interaction of adenosine triphosphate (ATP) with the P2X7 purinoceptor induces the secretion of IL-1beta and initiates the inflammatory response. In these experiments, that the addition of ATP to macrophages was found to induce P2X7 activation and secretion of IL-1beta. This secretion is enhanced with anti-RTF antibody in combination with exogenous ATP (p< 0.005). RTF is also revealed to be able to influence surface ATPase activity and, increase PI incorporation, which is an indicator of P2X7 activation. We demonstrate that RTF has a role in controlling IL-1beta secretion by regulating P2X7 activity.