Changes in Corneal Epithelial Layer Inflammatory Cells in Aqueous Tear-Deficient Dry Eye
Changes in Corneal Epithelial Layer Inflammatory Cells in Aqueous Tear-Deficient Dry Eye
复制标题
水性泪液缺乏性干眼症角膜上皮层炎症细胞的变化。
DOI：
10.1167/iovs.09-3629
复制
发表时间：
2010-01-01
影响因子：
4.4
通讯作者：
Liu, Zuguo
中科院分区：
文献类型：
--
作者：
Lin, Hui;Li, Wei;Liu, Zuguo
目的：观察32例非干燥综合征（non-Sjögren 'syndrome，NSS）干眼、14例干燥综合征（Sjögren' syndrome，SS）干眼及33例健康志愿者角膜上皮中炎性细胞的形态、分布及密度。应用激光扫描共聚焦显微镜观察角膜中央和周边上皮中朗格汉斯细胞（LCs）和白细胞的分布和密度。还评价了LC形态。结果：正常角膜上皮中央部（34.9 ± 5.7 cells/mm 2）和周边部（90.7 ± 8.2 cells/mm 2）均存在LC。此外，在NSS（89.8 +/- 10.8 cells/mm（2））和SS（127.9 +/- 23.7 cells/mm（2））患者的中央角膜上皮中，LC密度显著增加。干眼症组中突起明显的LC比例明显高于健康对照组。SS患者外周角膜上皮LC密度也增加，但NSS患者无此现象。正常角膜上皮中的白细胞密度非常低，而NSS的中央角膜上皮（4.6 +/- 1.0个细胞/mm（2））以及SS的中央（49.0 +/- 12.9个细胞/mm（2））和外周（84.2 +/- 36.8个细胞/mm（2））角膜上皮中的白细胞密度增加。LC和白细胞的密度表现出显着的相关性与临床evaluation.Conclusions中发现的严重程度：LC和白细胞在角膜上皮的变化表明他们参与水泪液缺乏性干眼症的病理生理。在体内动态评估中央角膜炎性细胞密度可作为干眼严重程度的指标，并为干眼治疗提供新的见解。
Purpose:To investigate the morphology, distribution, and density of inflammatory cells in the corneal epithelium of aqueous tear-deficient dry eye.Methods:Thirty-two patients with non-Sjögren's syndrome (NSS) dry eye, 14 patients with Sjögren's syndrome (SS) dry eye, and 33 healthy volunteers were studied. In vivo laser scanning confocal microscopy was used to investigate both Langerhans cell (LCs) and leukocyte distribution and density in the peripheral and central corneal epithelium. LC morphology was also evaluated. Multifactor regression analysis assessed whether there is a correlation between clinical manifestations and inflammatory cell densities.Results:LCs were present in both central (34.9 +/- 5.7 cells/mm(2)) and peripheral (90.7 +/- 8.2 cells/mm(2)) parts of the normal corneal epithelium. Moreover, LC density increased dramatically in the central corneal epithelium in patients with NSS (89.8 +/- 10.8 cells/mm(2)) and SS (127.9 +/- 23.7 cells/mm(2)). The ratio of LCs with obvious processes was much higher in patients with dry eye than in healthy volunteers. LC density also increased in peripheral corneal epithelium in patients with SS, but not in those with NSS. Leukocyte density in normal corneal epithelium was very low, whereas it increased in the central corneal epithelium (4.6 +/- 1.0 cells/mm(2)) in NSS and in both central (49.0 +/- 12.9 cells/mm(2)) and peripheral (84.2 +/- 36.8 cells/mm(2)) corneal epithelium in SS. Densities of LCs and leukocytes showed significant correlation with the severity found in clinical evaluation.Conclusions:The LC and leukocyte changes in the corneal epithelium suggest their involvement in aqueous tear-deficient dry eye pathophysiology. In vivo dynamic assessment of central corneal inflammatory cell density may serve as an indicator of dry eye severity and provide new insight for dry eye treatment.