Localized DNA Hybridization Chain Reactions on DNA Origami
Localized DNA Hybridization Chain Reactions on DNA Origami
复制标题
DOI：
10.1021/acsnano.7b06699
复制
发表时间：
2018-02-01
期刊：
影响因子：
17.1
通讯作者：
Reif, John
中科院分区：
文献类型：
--
作者：
Bui, Hieu;Shah, Shalin;Reif, John
DNA纳米科学领域已经展示了许多精致的DNA纳米结构和复杂的DNA纳米器件。然而，先前证明的DNA纳米器件的每个步骤的操作需要DNA链的扩散，并且这些器件的速度受到扩散动力学的限制。在这里，我们展示了自组装DNA折纸矩形表面上的局部DNA杂交反应链。我们的DNA纳米器件的定位设计不依赖于每个步骤的DNA链的扩散，从而提供更快的反应动力学。由于设备的本地化组件可以在其他位置重新使用，因此本地化还提供了相当大的可扩展性。各种技术，包括原子力显微镜，全内反射荧光，和合奏荧光光谱，用于确认发生局部DNA杂交反应的DNA折纸的表面上。我们的本地化DNA纳米器件有许多潜在的生物学应用，并且本地化设计可扩展到涉及在其他分子表面（例如细胞表面）上操作的DNA纳米器件的应用。
The field of DNA nanoscience has demonstrated many exquisite DNA nanostructures and intricate DNA nanodevices. However, the operation of each step of prior demonstrated DNA nanodevices requires the diffusion of DNA strands, and the speed of these devices is limited by diffusion kinetics. Here we demonstrate chains of localized DNA hybridization reactions on the surface of a self-assembled DNA origami rectangle. The localization design for our DNA nanodevices does not rely on the diffusion of DNA strands for each step, thus providing faster reaction kinetics. The locality also provides considerable increased scalability, since localized components of the devices can be reused in other locations. A variety of techniques, including atomic force microscopy, total internal reflection fluorescence, and ensemble fluorescence spectroscopy, are used to confirm the occurrence of localized DNA hybridization reactions on the surface of DNA origami. There are many potential biological applications for our localized DNA nanodevices, and the localization design is extensible to applications involving DNA nanodevices operating on other molecular surfaces, such as those of the cell.