CONFORMATIONAL PROPERTIES OF PHOSPHOLIPASES-A2 - SECONDARY-STRUCTURE PREDICTION, CIRCULAR-DICHROISM AND RELATIVE INTERFACE HYDROPHOBICITY
CONFORMATIONAL PROPERTIES OF PHOSPHOLIPASES-A2 - SECONDARY-STRUCTURE PREDICTION, CIRCULAR-DICHROISM AND RELATIVE INTERFACE HYDROPHOBICITY
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DOI：
10.1111/j.1432-1033.1983.tb07859.x
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
HIDER, RC
中科院分区：
文献类型：
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作者：
DUFTON, MJ;EAKER, D;HIDER, RC
对32种磷脂酶A2（哺乳动物、眼镜蛇、蝰蛇）的二级结构进行了预测，并与已有的晶体学数据进行了比较。很好的协议是显而易见的预测和实验之间，特别是螺旋结构。圆二色性光谱也确定了6酶从眼镜蛇蛇毒和这些，与以前发表的光谱，确认的主要含义的预测，即所有的同系物具有定性相似的三级结构。然后根据上述调查结果考虑了可能的结构/活动关系。预测了被认为与脂质/水界面相互作用的酶区域的相对疏水性/亲水性，并注意到与相对穿透能力、来源物种和β-葡聚糖的存在的某些相关性。神经毒性
The sequences of 32 phospholipases A2 [mammalian, Elapid snakes, Viperid snakes] were anlayzed by secondary-structure prediction and the results were compared with the available crystallographic data. Good agreement is evident between prediction and experiment, especially for helical structure. Circular dichroic spectra were also determined for 6 enzymes from Elapid snake venom and these, in association with previously published spectra, confirm the main implication of the predictions, namely that all the homologues have qualitatively similar tertiary structures. Consideration was then given to possible structure/activity relationships in the light of the above findings. The relative hydrophobicity/hydrophilicity of the area of the enzyme thought to interact with lipid/water interfaces was predicted and certain correlations were noted with relative penetrating power, species of origin and the presence of .beta.-neurotoxic properties.