Liquid–Liquid Extraction of Insecticides from Juice: An Analytical Chemistry Laboratory Experiment
Liquid–Liquid Extraction of Insecticides from Juice: An Analytical Chemistry Laboratory Experiment
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从果汁中液液萃取杀虫剂：分析化学实验室实验
DOI：
10.1021/ed300389p
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发表时间：
2013
影响因子：
3
通讯作者：
P. Ryan
中科院分区：
文献类型：
--
作者：
Samantha A Radford;Ronald E. Hunter;D. Barr;P. Ryan
一个实验室实验是针对分析化学的学生，教他们有关食品中的杀虫剂，样品提取和清理。使用液-液萃取法提取加入到苹果汁样品中的12种杀虫剂的微量浓度（低于μg/mL水平），并使用伯仲胺（PSA）柱或C18柱进行净化。然后通过气相色谱法分离提取物，并采用串联质谱法进行分析。该方法已成功地测试了三个队列的定量分析的学生，以确定在50 ng/mL的苹果汁中的杀虫剂的浓度。使用C18测试卡片（n = 30），学生的平均回收率在78%至121%之间。使用PSA检测盒，平均回收率在47 - 121%之间（n = 6）。总的来说，这个实验室实验是成功的教学分析化学学生环境暴露于杀虫剂和样品处理。
A laboratory experiment was developed to target analytical chemistry students and to teach them about insecticides in food, sample extraction, and cleanup. Micro concentrations (sub-μg/mL levels) of 12 insecticides spiked into apple juice samples are extracted using liquid–liquid extraction and cleaned up using either a primary–secondary amine (PSA) cartridge or a C18 cartridge. Extracts are then separated by gas chromatography and analyzed with tandem mass spectrometry. The method has been successfully tested by three cohorts of quantitative analysis students to determine the concentration of the insecticides spiked at 50 ng/mL in apple juice. Students’ average recoveries were between 78 and 121% using C18 cartridges (n = 30). Using PSA cartridges, average recoveries were between 47 and 121% (n = 6). Overall, this laboratory experiment was successful in teaching analytical chemistry students about environmental exposure to insecticides and sample processing.