Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
Importance of the lipid peroxidation biomarkers and methodological aspects FOR malondialdehyde quantification
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DOI：
10.1590/s0100-40422009000100032
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发表时间：
2009-01-01
期刊：
影响因子：
--
通讯作者：
Farina, Marcelo
中科院分区：
文献类型：
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作者：
Grotto, Denise;Maria, Lucas Santa;Farina, Marcelo
自由基引起脂质过氧化，在病理过程中起重要作用。自由基介导的损伤可以通过共轭二烯、丙二醛、4-羟基壬烯醛等来测量。然而，丙二醛已被指出是评价脂质过氧化的主要产物。大多数测定法通过丙二醛与硫代巴比妥酸的反应来测定丙二醛，硫代巴比妥酸可以通过间接（光谱法）和直接方法（色谱法）来测量。虽然有一些争议的方法，选择性HPLC为基础的测定提供了一个更可靠的脂质过氧化措施。本文综述了MDA测定的重要方面，在病理学和生物样品处理中的重要性。
Free radicals induce lipid peroxidation, playing an important role in pathological processes. The injury mediated by free radicals can be measured by conjugated dienes, malondialdehyde, 4-hydroxynonenal, and others. However, malondialdehyde has been pointed out as the main product to evaluate lipid peroxidation. Most assays determine malondialdehyde by its reaction with thiobarbituric acid, which can be measured by indirect (spectrometry) and direct methodologies (chromatography). Though there is some controversy among the methodologies, the selective HPLC-based assays provide a more reliable lipid peroxidation measure. This review describes significant aspects about MDA determination, its importance in pathologies and biological samples treatment.